This kit provides a simple and fast solution for the extraction of circulating nuclear acid from serum, 電漿, and other cell-free liquid samples. Circulating nucleic acid refers to the free extracellular nucleic acid produced by cell apoptosis, of which fragments are generally below 1KB. The kit is based on silica gel column purification technology, which is no need for toxic phenol chloroform extraction and time-consuming alcohol precipitation during the extraction. The obtained Circulating Nucleic Acid can be directly used for quantitative 聚合酶鍊式反應, liquid or solid phase chip analysis, 雜交, 和SNP檢測.
HiPure Circulating DNA/RNA Kit adopts a unique solution system and multiple layers of filter membranes with different pore sizes, which can efficiently process large volumes of serum and plasma samples and capture extremely small amounts of free nucleic acids.
細節
規格
| 特徵 | 規格 |
| 主要功能 | 分離循環 DNA/RNA (包括 miRNA) 從 1-5 毫升血清和血漿 |
| 應用領域 | 定量PCR / 逆轉錄聚合酶連鎖反應, liquid or solid-phasechip analysis, 雜交和SNP檢測 |
| 純化方法 | Midi 旋轉柱 |
| 淨化技術 | 二氧化矽技術, DNA filtration technology |
| 工藝方法 | 手動的 (離心或真空) |
| 樣品類型 | 血清, 電漿, and other cell-free liquid samples |
| 樣品量 | 1-5 毫升 |
| 洗脫體積 | ≥20μl |
| 每次運行時間 | ≤100 minutes |
| 每根柱的載液量 | 4 毫升 |
| 柱結合率 | 1 毫克 |
原則
This kit is based on silica gel column technology. Serum or other liquid samples are lysed and digested in buffer CFL. After adding buffer CFP, the protein is removed by centrifugation to obtain the supernatant. Isopropanol is added to precipitate the total nucleic acid and transferred to the column for filtration. 脫氧核糖核酸 / RNA is adsorbed on the membrane of the column, while the protein is not adsorbed and removed with the filtrate.The column is washed with buffer MGW1 to remove protein and other impurities, and then washed with buffer RW2 to remove salt. 最後, 脫氧核糖核酸 / RNA is eluted by low salt buffer. The eluted DNA / RNA can be directly used for quantitative PCR/ RT-PCR, 液相或固相晶片分析, 雜交和SNP檢測.
優點
- 高產 – most optimized process to obtain maximum free RNA and small RNA
- 高濃度 – 低洗脫體積 (>20微升) to ensure nucleic acid concentration
- 高純度 – low alcohol combination, completely remove inhibitor and protein pollution
- 高回收率 – silica gel column technology can recover nucleic acid molecules at the level of PG
- Large volume – 1-2ml serum and plasma samples can be processed at one time
套件內容
| 內容 | R431602 | D431603 |
| 淨化時間 | 50 準備工作 | 250 準備工作 |
| Hipure RNA微柱 | 50 | 5 X 50 |
| HiPure Viral Midi Columns | 50 | 5 X 50 |
| 15 毫升收集管 | 50 | 5 X 50 |
| 2毫升收集管 | 50 | 5 X 50 |
| Buffer CFL | 150 毫升 | 2 X 375 毫升 |
| Buffer CFP | 30 毫升 | 150 毫升 |
| Buffer MGW1* | 100 毫升 | 2 X 250 毫升 |
| 緩衝器 RW2* | 2 X 50 毫升 | 5 X 100 毫升 |
| 無核糖核酸酶水 | 10 毫升 | 50 毫升 |
儲存和穩定性
The kit components can be stored at room temperature (15–25°C) 並且至少穩定 18 在這些條件下幾個月.
評論
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