CleanSeq 微珠

介紹

• 科技: Utilizes paramagnetic bead technology for rapid and high-performance dye-terminator removal.
• No Centrifugation or Filtration: Eliminates the need for centrifugation or filtration steps, simplifying the purification process.
• Manual or Automated: This can be performed manually or fully automated for high-throughput applications, providing flexibility in workflow.
• Improved Sequencing Results: Produces sequences with longer Phred 20 read lengths and higher signal intensities compared to similar purification systems.
• Enhanced Performance: Offers superior performance in terms of sequence quality and signal intensity, enhancing downstream analysis and interpretation.

規格

特徵	規格
主要功能	Removal of free fluorescent dye from sequencing solution (Replace Beckmen or Agincourt CleanSeq)
應用領域	Automated sequences
淨化技術	磁珠技術
工藝方法	手動或自動
樣品類型	DNA doped with fluorescent dye
樣品量	5微升
恢復	90%
洗脫體積	≥25μl
運行時間	≤50分鐘

原則

CleanSeq offers a rapid and efficient method for purifying sequencing reaction products, ideal for applications like Sanger sequencing.

• Magnetic Bead Technology: Utilizes magnetic particles within an optimized binding buffer to selectively capture desired DNA fragments.
• In-Plate Purification: The entire protocol can be performed directly in the thermal cycling plate, eliminating the need for transfer steps.
• Efficient Cleanup: Removes unincorporated dyes, 核苷酸, 鹽類, and contaminants through a simple washing procedure.
• Automation Friendly: No centrifugation or filtration is required, making CleanSeq compatible with various automated liquid handling platforms.

This streamlined approach minimizes processing time and simplifies workflow, making CleanSeq a valuable tool for high-throughput sequencing applications.

優點

• 高回收率 – 取決於 90%
• 高通量 – 採用磁珠​​純化技術
• 簡單的 – 簡化操作流程, 組合 – 洗滌 – 洗脫

套件內容

內容	BCS-5	BCS-50	BCS-500
CleanSeq 微珠	5 毫升	50 毫升	500 毫升

儲存和穩定性

貯存:

• 為了獲得最佳性能, store CleanSeq Beads at 2-8°C (冰箱) upon arrival and up to 6 月.
• 短期儲存 (取決於 12 週) 在室溫下 (15-25℃) 在不影響性能的情況下可以接受.

Before Use:

• Ensure proper mixing of CleanSeq Beads before each use.
• The reagent should appear homogenous and consistent in color throughout.

不要凍結