介紹
- 科技: Utilizes paramagnetic bead technology for rapid and high-performance dye-terminator removal.
- No Centrifugation or Filtration: Eliminates the need for centrifugation or filtration steps, simplifying the purification process.
- Manual or Automated: This can be performed manually or fully automated for high-throughput applications, providing flexibility in workflow.
- Improved Sequencing Results: Produces sequences with longer Phred 20 read lengths and higher signal intensities compared to similar purification systems.
- Enhanced Performance: Offers superior performance in terms of sequence quality and signal intensity, enhancing downstream analysis and interpretation.
規格
特徵 | 規格 |
主要功能 | Removal of free fluorescent dye from sequencing solution (Replace Beckmen or Agincourt CleanSeq) |
應用領域 | Automated sequences |
淨化技術 | 磁珠技術 |
工藝方法 | 手動或自動 |
樣品類型 | DNA doped with fluorescent dye |
樣品量 | 5微升 |
恢復 | 90% |
洗脫體積 | ≥25μl |
運行時間 | ≤50分鐘 |
原則
CleanSeq offers a rapid and efficient method for purifying sequencing reaction products, ideal for applications like Sanger sequencing.
- Magnetic Bead Technology: Utilizes magnetic particles within an optimized binding buffer to selectively capture desired DNA fragments.
- In-Plate Purification: The entire protocol can be performed directly in the thermal cycling plate, eliminating the need for transfer steps.
- Efficient Cleanup: Removes unincorporated dyes, 核苷酸, 鹽類, and contaminants through a simple washing procedure.
- Automation Friendly: No centrifugation or filtration is required, making CleanSeq compatible with various automated liquid handling platforms.
This streamlined approach minimizes processing time and simplifies workflow, making CleanSeq a valuable tool for high-throughput sequencing applications.
優點
- 高回收率 – 取決於 90%
- 高通量 – 採用磁珠純化技術
- 簡單的 – simplified operation process, combination – 洗滌 – elution
套件內容
內容 | BCS-5 | BCS-50 | BCS-500 |
CleanSeq 微珠 | 5 毫升 | 50 毫升 | 500 毫升 |
儲存和穩定性
貯存:
- 為了獲得最佳性能, store CleanSeq Beads at 2-8°C (冰箱) upon arrival and up to 6 月.
- 短期儲存 (取決於 12 週) 在室溫下 (15-25℃) 在不影響性能的情況下可以接受.
Before Use:
- Ensure proper mixing of CleanSeq Beads before each use.
- The reagent should appear homogenous and consistent in color throughout.
不要凍結
評論
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