Solarbio Von Kossa Calcium Stain Kit

ส่วนประกอบชุดอุปกรณ์

การแนะนำ

Calcium is abundant in the human body, forming bones as a scaffold to support it. Calcium plays a crucial role in secretion, การขนส่ง, muscle contraction, nerve conduction, และอื่น ๆ. Several dyes can form chelates with calcium, such as Alizarin Red 5, Purpurin, Nuclear Fast Red, ฯลฯ. Alizarin Red S, a sodium alizarin sulfonate, is an anthraquinone derivative capable of chelating with calcium carbonate or calcium phosphate to form an orange-red complex. Common methods for calcium salt staining include the Von Kossa method and the Alizarin Red S method. This kit’s principle relies on a metal replacement method: when Von Kossa Silver Solution acts on sections containing insoluble calcium salt, calcium is replaced by silver, and the silver salt reduces to black metal silver under light exposure. It’s suitable for calcium salt tissue staining of numerous samples.

มาตรการ (สำหรับการอ้างอิงเท่านั้น)

1. Fix the tissue with 10% NBF and conventionally dehydrate and embed.
2. Cut the section into 5µm, conventionally dewax and hydrate.
3. Dye the section with Von Kossa Silver Solution under strong light for 15–60 minutes.
4. Rinse with distilled water for 1 นาที. Treat with Hypo Solution for 2 นาที.
5. (Optional) Re-dyeing with HE solution or Van Gieson Solution.
6. Conventionally dehydrate, transparent, and seal with resinene.

การควบคุมเชิงลบ (optional)

Take another adjacent section and dewax it to water. Put it into Von Kossa Control Solution for 20 นาที, then rinse it with running water for 5 นาที. ต่อมา, place it into Von Kossa Silver Solution together with the experimental section, and follow the same steps as above.

ผลลัพธ์

• Calcium Salt: Brownish Red to Dark Black
• นิวเคลียส: Range with re-dyeing solution
• Background: Range with re-dyeing solution

บันทึก

1. It’s best to use NBF for fixing tissues containing calcium salt.
2. The action time depends on the intensity and duration of light exposure.
3. If exposed to UV light, reduce exposure time to 5-10 นาที.
4. เพื่อความปลอดภัยและสุขภาพ, wear experimental clothing and disposable gloves