Introduction
- Extracts DNA from various sources: tissue, cells, blood, saliva, swabs, blood spots, semen, and other clinical samples.
- Fast and easy to use.
- Purified DNA can be used for PCR, qPCR, Southern Blot, viral DNA testing, and more.
Specifications
| Features | Specifications |
| Main Functions | Isolation of total DNA from 1-100ul blood, FFPE, tissue, and other samples |
| Applications | Second-generation sequencing, PCR, real-time PCR, etc. |
| Purification method | Monodisperse magnetic beads |
| Purification technology | Magnetic beads technology |
| Process method | Manual or automatic |
| Sample type | Anticoagulant blood, concentrated blood, buffy coat, lymphocytes, and cultured cells |
| Sample amount | Body fluid : 10 – 200μl, Tissue : ≤10mg |
| Yield | 10ng – 15μg |
| Elution volume | 80 -100μl |
| Time per run |
Principle
- Utilizes high-binding magnetic particles for purification, ensuring efficient DNA isolation.
- The sample undergoes lysis and digestion facilitated by lysate and Protease, releasing DNA into the solution.
- Magnetic particles and binding solution are introduced, causing DNA to adhere to particle surfaces while impurities such as proteins are left unbound.
- Wash with a specialized solution to remove proteins and impurities, followed by ethanol wash to eliminate salts.
- DNA is ultimately eluted using an Elution Buffer, providing purified DNA ready for downstream applications.
Advantage
- High binding capability – ideal for processing DNA-rich samples like whole blood, buffy coat, and concentrated blood.
- Exceptional purity – features carboxyl magnetic beads with minimal surface adsorption, ensuring superior purity.
- Versatile – applicable across a wide range of clinical samples, offering general utility.
Kit Contents
| Contents | IVD3101 |
| Purification Times | 200 |
| MagBind Particles | 4.5 ml |
| Proteinase K | 90 mg |
| Protease Dissolve Buffer | 10 ml |
| Buffer ATL | 60 ml |
| Buffer AL | 60 ml |
| Buffer BD* | 20 ml |
| Buffer BXW1* | 110 ml |
| Elution Buffer | 30 ml |
Storage and Stability
- Proteinase K and MagBind Particles should be stored at 2–8°C upon receipt.
- Short-term storage (up to 18 weeks) at room temperature (15–25°C) does not compromise their performance.
- The remaining components can be stored dry at room temperature (15–25°C) and remain stable for at least 18 months under these conditions.
