HiPure FFPE DNA Kit

The HiPure FFPE Nucleic Acid Kit offers a fast and user-friendly solution for extracting DNA from formalin-fixed, paraffin-embedded (FFPE) tissue samples.

• Target Samples: FFPE tissue and sections
• Simple and Rapid: DNA extraction can be completed in just 20 minutes (excluding digestion time).
• Silica Gel Technology: Employs a reliable silica gel column purification method, eliminating the need for harsh organic solvents (phenol-chloroform) or alcohol precipitation steps.
• Downstream Applications: Purified DNA is suitable for various analyses like PCR (polymerase chain reaction), Southern blotting, and viral DNA detection.

Benefits:

• Streamlined Workflow: The silica gel column technology offers a faster and less hazardous alternative to traditional methods.
• High-Quality DNA: The extraction process ensures the isolation of DNA suitable for diverse downstream applications.
• Broad Applicability: The kit works effectively with various FFPE tissue samples.

This kit empowers researchers working in various fields, including oncology, pathology, and infectious diseases, to efficiently extract DNA from archived FFPE samples for further analysis.

Specifications

Features	Specifications
Main Functions	Isolation of total DNA from FFPE tissue samples
Applications	PCR, southern blot viral DNA detection, etc.
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Formalin-fixed, paraffin-embedded (FFPE) tissue and section samples
Sample amount	<20mg
Elution volume	<20µl
Time per run	<span “=”” style=”outline: 0px; box-sizing: border-box; font-size: 16px;”>≤20 minutes
Liquid carrying volume per column	4ml
Binding yield of column	100μg

Principle

HiPure FFPE Nucleic Acid Kit, designed for rapid and efficient extraction of both DNA and RNA from formalin-fixed, paraffin-embedded (FFPE) tissue samples:

Key Features:

• Silica Gel Column Technology: Utilizes a reliable and streamlined approach, eliminating the need for harsh chemicals like phenol-chloroform.
• Fast Processing: Extracts DNA/RNA in just 20 minutes, excluding digestion time.
• Sample Compatibility: Works effectively with various FFPE tissue sections.

Extraction Process:

1. Deparaffinization: Xylene removes paraffin wax, a common component used to preserve tissues.
2. Lysis and Digestion: A special solution (lysate) and an enzyme (protease) break down cells and proteins, releasing nucleic acids (DNA and RNA) into the solution.
3. De-Crosslinking: Heating at 90°C reverses the effects of formalin fixation, allowing easier access to nucleic acids.
4. Binding to Silica Membrane: After adjusting conditions with ethanol, the sample is transferred to a column. DNA/RNA binds to the silica membrane, while proteins pass through.
5. Washing: Buffers remove impurities like proteins and salts from the captured nucleic acids.
6. Elution: A low-salt buffer elutes the purified DNA/RNA, making it ready for downstream applications.

Applications:

• PCR (polymerase chain reaction)
• Southern blotting
• Viral nucleic acid detection
• Other analyses requiring high-quality DNA or RNA

Advantages

The HiPure FFPE Nucleic Acid Kit offers a superior solution for extracting DNA and RNA from formalin-fixed, paraffin-embedded (FFPE) tissue samples.

Safety:

• No Xylene Contact: Eliminates the need for hazardous xylene, minimizing exposure to harmful chemicals.

Speed:

• Fast Processing: Extracts DNA/RNA in just 20 minutes (excluding digestion time), allowing you to process multiple samples within 2 hours.
• No Overnight Incubation: Streamlines the workflow by eliminating the need for lengthy overnight incubation steps.

High Efficiency:

• Enhanced PCR Sensitivity: Effectively removes formaldehyde modifications from DNA, significantly improving the sensitivity of downstream PCR (polymerase chain reaction) analyses.

Kit Contents

Contents	D312602	D312603
Purification Times	50 Preps	250 Preps
HiPure DNA Mini Columns I	50	250
2ml Collection Tubes	50	250
Buffer DPS	30 ml	150 ml
Buffer ATL	15 ml	60 ml
Buffer AL	15 ml	60 ml
Buffer GW1*	22 ml	88 ml
Buffer GW2*	12 ml	50 ml
Proteinase K	24 mg	120 mg
Protease Dissolve Buffer	1.8 ml	10 ml
Buffer AE	10 ml	30 ml

Storage and Stability

• Proteinase K: Keep it cool in the refrigerator (2-8°C) upon arrival for maximum effectiveness.
• Short-Term Storage (up to 12 weeks): Proteinase K can last at room temperature (15-25°C) for up to 12 weeks without issues.
• Everything Else: Store other kit components at room temperature (15-25°C). They are stable for at least 18 months under these conditions.
• Important: If storing the entire kit cold, remember to warm up the buffers before use. All buffers should be at room temperature for optimal results.

Experiment Data