Introduction
- Technology: Utilizes paramagnetic bead technology for rapid and high-performance dye-terminator removal.
- No Centrifugation or Filtration: Eliminates the need for centrifugation or filtration steps, simplifying the purification process.
- Manual or Automated: This can be performed manually or fully automated for high-throughput applications, providing flexibility in workflow.
- Improved Sequencing Results: Produces sequences with longer Phred 20 read lengths and higher signal intensities compared to similar purification systems.
- Enhanced Performance: Offers superior performance in terms of sequence quality and signal intensity, enhancing downstream analysis and interpretation.
Specifications
| Features | Specifications |
| Main Functions | Removal of free fluorescent dye from sequencing solution (Replace Beckmen or Agincourt CleanSeq) |
| Applications | Automated sequences |
| Purification technology | Magnetic beads technology |
| Process method | Manual or automatic |
| Sample type | DNA doped with fluorescent dye |
| Sample amount | 5μl |
| Recovery | 90% |
| Elution volume | ≥25μl |
| Operation time | ≤50 minutes |
Principle
CleanSeq offers a rapid and efficient method for purifying sequencing reaction products, ideal for applications like Sanger sequencing.
- Magnetic Bead Technology: Utilizes magnetic particles within an optimized binding buffer to selectively capture desired DNA fragments.
- In-Plate Purification: The entire protocol can be performed directly in the thermal cycling plate, eliminating the need for transfer steps.
- Efficient Cleanup: Removes unincorporated dyes, nucleotides, salts, and contaminants through a simple washing procedure.
- Automation Friendly: No centrifugation or filtration is required, making CleanSeq compatible with various automated liquid handling platforms.
This streamlined approach minimizes processing time and simplifies workflow, making CleanSeq a valuable tool for high-throughput sequencing applications.
Advantages
- High recovery – up to 90%
- High throughput – using magnetic beads purification technology
- Simple – simplified operation process, combination – washing – elution
Kit Contents
| Contents | BCS-5 | BCS-50 | BCS-500 |
| CleanSeq Beads | 5 ml | 50 ml | 500 ml |
Storage and Stability
Storage:
- For optimal performance, store CleanSeq Beads at 2-8°C (refrigerator) upon arrival and up to 6 months.
- Short-term storage (up to 12 weeks) at room temperature (15-25°C) is acceptable without affecting performance.
Before Use:
- Ensure proper mixing of CleanSeq Beads before each use.
- The reagent should appear homogenous and consistent in color throughout.
DO NOT FREEZE
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