HiPure 토양/대변 DNA 96 전부

• Isolation of High-Quality Genomic DNA: The product enables rapid and reliable isolation of high-quality genomic DNA from various soil and stool samples.
• Sample Processing Capacity: Capable of processing up to 100mg of stool samples and 500 mg of soil samples within 60 분.
• Innovative System Design: Combines the reversible nucleic acid binding properties of HiPure matrix with the speed and versatility of 스핀 컬럼 기술.
• Elimination of PCR Inhibitors: Effectively eliminates PCR-inhibiting compounds such as humic acid from soil samples during the purification process.
• Suitability for Various Applications: The purified DNA is suitable for PCR, restriction digestion, 및 차세대 시퀀싱.
• Reduction in Organic Extractions: No organic extractions are required, thus reducing plastic waste and hands-on time.
• Increased Efficiency: Allows for the processing of multiple samples in parallel, enhancing overall workflow efficiency.

명세서

원칙

• Sample Homogenization and Lysis: Soil or stool samples are homogenized and treated in a specially formulated buffer containing detergent. This process effectively lyses bacteria, 누룩, 그리고 곰팡이 샘플.
• Removal of Contaminants: Our proprietary Absorber Solution is utilized to remove humic acid, 단백질, 다당류, and other contaminants from the lysed sample.
• DNA Binding and Column Application: After adjusting binding conditions, the treated sample is applied to a DNA Mini Column, facilitating the binding of DNA to the column matrix.
• Rapid Wash Steps: Two rapid wash steps are employed to remove trace contaminants, ensuring the purity of the extracted DNA.
• Elution of Purified DNA: Pure DNA is eluted from the column in a low ionic strength buffer, providing high-quality, purified DNA ready for downstream applications.
• Direct Use in Downstream Applications: The purified DNA can be directly used in downstream applications without the need for further purification steps, streamlining laboratory workflows and saving time.

장점

• 빠른 – 여러 샘플을 추출할 수 있습니다. 40 분 (소화 후)
• 고순도 – the purified DNA can be directly used in various downstream applications
• 좋은 반복성 – 실리카 기술은 매번 이상적인 결과를 얻을 수 있습니다
• 높은 회복력 – PG 수준에서 DNA 복구 가능

키트 내용물

보관 및 안정성

• Absorber Solution Storage: 도착 시, the Absorber Solution should be stored at 2-8°C. 하지만, it can also be stored at room temperature (15-25℃) for short-term periods (까지 24 주) without affecting its performance.
• 나머지 키트 구성 요소: The remaining components of the kit can be stored dry at room temperature (15-25℃). They remain stable for at least 18 이런 조건에서 몇 달 동안, ensuring their efficacy and performance over an extended period.