HiPure 플라스미드 EF 맥시 키트

소개

HiPure Plasmid EF Maxi Kit Features:

1. Technology Integration:
   • The kit integrates HiPure technology for plasmid purification with Magen’s innovative Endotoxin Removal Technology (ETR).
2. Endotoxin Removal:
   • Specifically designed to achieve low levels of endotoxins in the purified 플라스미드 DNA, enhancing its suitability for sensitive applications.
3. Application Suitability:
   • Ideal for use in eukaryotic transfection and various in vitro experiments due to the high purity and low endotoxin levels of the DNA.
4. Capacity and Yield:
   • Capable of purifying up to 1000 µg of high copy number plasmid DNA or 200 µg of low copy number plasmid DNA.
5. Culture Volume Processing:
   • Efficient purification from up to 150 mL of an overnight bacterial culture.

세부

명세서

원칙

HiPure Plasmid Procedure:

1. Alkaline Lysis and DNA Adsorption:
   • Based on alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt.
   • The unique silica membrane in the kit replaces glass or silica slurries for plasmid DNA minipreps.
2. 세 가지 기본 단계:
   • 단계 1: Preparation and clearing of a bacterial lysate using the alkaline method.
   • 단계 2: Transfer of the supernatant to a column for DNA binding.
   • 단계 3: 단백질 및 기타 불순물을 제거하기 위해 세척, followed by elution of nucleic acid with low-salt buffer (10 mm 트리스, pH 9.0, 0.5 mm EDTA).

장점

• High purity — purified plasmid can be directly used in sequencing, 효소 소화 및 PCR, 등.
• Fast — silica gel column purification is much faster than ion exchange
• High yield — up to 1.5 mg plasmid can be binded in one column
• Ultra low endotoxin — content of endotoxin is less than 0.05 EU/μg, which can be directly used for cell transfection and animal injection, 등.

키트 내용물

보관 및 안정성

Storage Instructions:

1. General Storage:
   • 키트 구성 요소는 실온에서 건조 할 수 있습니다 (15–25 °C).
   • 적어도 안정적입니다 18 이런 조건에서 몇 달 동안.
2. 완충액은 침전됩니다:
   • 완충액에 침전물이 생기는 경우, warm at 37°C to dissolve.
3. Specific Storage for RNase A and Buffer P1:
   • RNase A and Buffer P1 are stable for 6 months when stored at 2–8 °C.

구매안내