CleanSeq 비드

소개

• 기술: Utilizes paramagnetic bead technology for rapid and high-performance dye-terminator removal.
• No Centrifugation or Filtration: Eliminates the need for centrifugation or filtration steps, simplifying the purification process.
• Manual or Automated: This can be performed manually or fully automated for high-throughput applications, providing flexibility in workflow.
• Improved Sequencing Results: Produces sequences with longer Phred 20 read lengths and higher signal intensities compared to similar purification systems.
• Enhanced Performance: Offers superior performance in terms of sequence quality and signal intensity, enhancing downstream analysis and interpretation.

명세서

원칙

CleanSeq offers a rapid and efficient method for purifying sequencing reaction products, ideal for applications like Sanger sequencing.

• 마그네틱 비드 기술: Utilizes magnetic particles within an optimized binding buffer to selectively capture desired DNA fragments.
• In-Plate Purification: The entire protocol can be performed directly in the thermal cycling plate, eliminating the need for transfer steps.
• Efficient Cleanup: Removes unincorporated dyes, 뉴클레오티드, 염류, and contaminants through a simple washing procedure.
• Automation Friendly: No centrifugation or filtration is required, making CleanSeq compatible with various automated liquid handling platforms.

This streamlined approach minimizes processing time and simplifies workflow, making CleanSeq a valuable tool for high-throughput sequencing applications.

장점

• 높은 회복력 – 까지 90%
• 높은 처리량 – using magnetic beads purification technology
• 단순한 – simplified operation process, combination – 세탁 – elution

키트 내용물

보관 및 안정성

저장:

• 최적의 성능을 위해, store CleanSeq Beads at 2-8°C (냉장고) upon arrival and up to 6 개월.
• 단기 보관 (까지 12 주) 실온에서 (15-25℃) 성능에 영향을 주지 않고 허용 가능.

Before Use:

• Ensure proper mixing of CleanSeq Beads before each use.
• The reagent should appear homogenous and consistent in color throughout.

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