ソーラーバイオ BCA プロテインアッセイキット 50T

BCA (ビシンコニン酸) Protein Assay Kit is a widely utilized colorimetric method for the quantification of protein concentrations in a sample. This assay relies on the reduction of Cu^2+ ions to Cu^1+ by proteins under alkaline conditions, forming a purple-colored complex with bicinchoninic acid. The intensity of the color is directly proportional to the protein concentration, 正確な定量化を可能にする.

Here is a step-by-step guide on how to use a BCAプロテイン アッセイキット:

必要な材料:

1. BCAタンパク質アッセイキット
2. Protein standards (bovine serum albumin is commonly used)
3. Sample containing unknown protein concentrations
4. マイクロプレートまたはキュベット
5. マイクロピペットとチップ
6. マイクロプレートリーダーまたは分光光度計
7. 蒸留水

手順:

1. タンパク質標準物質の調製:
   • 既知の濃度で一連のタンパク質標準物質を調製します. これらの標準は、定量用の標準曲線を作成するために使用されます。. Common concentrations are 0, 0.125, 0.25, 0.5, 0.75, そして 1.0 mg/mL.
2. Preparation of Sample:
   • Dilute your protein sample to a suitable concentration within the linear range of the assay. If needed, you may need to perform a preliminary dilution to ensure that the sample falls within the standard curve.
3. BCA作用試薬の調製:
   • Mix BCA Reagent A and BCA Reagent B in a 50:1 比率 (typically provided in the kit). 完全に混合してください.
4. Addition of Standards and Sample:
   • Pipette aliquots of the protein standards and the sample into separate wells of a microplate. Each standard and sample should be run in duplicate or triplicate.
5. Addition of BCA Working Reagent:
   • Add an appropriate volume of the BCA Working Reagent to each well containing standards and samples. 十分に混ぜ合わせてください.
6. インキュベーション:
   • Incubate the microplate at an appropriate temperature (typically 37°C) のために 30 minutes to allow the color reaction to occur.
7. 測定:
   • Measure the absorbance of each well at 562 マイクロプレートリーダーまたは分光光度計を使用したnm.
8. 標準曲線の作成:
   • 標準の吸光度を既知の濃度に対してプロットして、標準曲線を作成します。.
9. Quantification of Sample:
   • Use the standard curve to determine the protein concentration of your sample based on its absorbance.
10. データ分析:
    • Calculate the concentration of protein in the sample based on the standard curve. Most microplate readers have software that can assist in this analysis.

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