マグピュア粒子N

導入

Magnetic bead nucleic acid purification technology uses nano or micron superparamagnetic material as the matrix, generally black ferric oxide or yellowish-brown ferric oxide as the magnetic material. The surface of the bead is coated with appropriate functional groups, which can adsorb nucleic acid. Magnetic beads are commonly used for nucleic acids, containing carboxyl groups, hydroxyl groups, またはシリコングループ. シリコンベースの磁気ビーズが最も一般的です, and their principle of adsorbing nucleic acid is consistent with the classical glass milk purification technology or glass fiber filter membrane purification method. Magpure particle is a kind of polydisperse fast-speed silica magnetic beads. The core is ferric oxide, accounting for 50%, and the surface coating is silica, accounting for 50%. The product can be used for plasmid extraction, ゲルDNA回復, 製品浄化, ゲノムDNAおよび RNA抽出, and viral nucleic acid extraction.

詳細

仕様

原理

Highsalt mediated binding: in the solution containing 2-4M guanidine isothiocyanate, 大型粒子 can selectively recover DNA molecules, and impurities such as protein polysaccharides are not adsorbed.

アルコール媒介結合: in the solution containing guanidine salt and alcohol (>25%), Magpure particles can selectively recover DNA/RNA molecules, and proteins and other impurities are not adsorbed.

After biological samples are treated with a digestive solution or lysis Buffer, DNA/RNA is released from cells, オルガネラ, およびタンパク質複合体 (リボソームとヌクレオソーム) into reagents. After Magpure particles and binding solution are added, DNA/RNA is adsorbed to the surface of Magpure particles to form the DNA/RNA bead complex. Under the action of the magnetic field, the magnetic beads are separated and collected, and the impurities such as protein are removed with the waste liquid. After two or three steps of further cleaning, DNA/RNA磁気ビーズ複合体は、滅菌水またはTEバッファーに再懸濁されます, and the DNA/RNA falls off from the surface of the magnetic beads, to achieve the purpose of purification.

注文情報

購入ガイド

• The MagPure magnetic-particle technology combines the speed and efficiency of silica-based DNA purification with the convenient handling of magnetic particles. DNAは、カオトロピック塩の存在下で磁気粒子のシリカ表面に結合します. DNA bound to the particles is then efficiently washed, considerably improving the purity of DNA. High-quality DNA is eluted. The automated purification procedure completely removes enzymes, ヌクレオチド, and other contaminants and inhibitors. Purified DNA is suitable for direct use in downstream applications, such as sequencing and microarray analysis.