導入
This product offers a fast and reliable method to isolate RNA, miRNAを含む, from a wide range of samples:
- 組織
- 細胞
- 血
- Other clinical samples
The extracted RNA is suitable for various downstream applications, 含む:
- RT-PCR (Reverse Transcription PCR)
- Quantitative RT-PCR (QRT-PCR)
- Viral RNA detection
- Other RNA-based analyses
This highlights the product’s versatility and compatibility with various research needs in gene expression analysis and RNA detection.
仕様
| 特徴 | 仕様 |
| 主な機能 | Isolation of total RNA（miRNA）from tissue, and cell using two columns and DNase plus reagent |
| アプリケーション | RT-PCR, cDNA合成, second-generation sequencing |
| 製品 | RNA, miRNA |
| 精製方法 | ミニ スピンカラム |
| 精製技術 | シリカ技術 |
| 加工方法 | マニュアル (遠心分離または真空) |
| サンプルの種類 | Clinical tissues, 細胞, リンパ球 |
| サンプル量 | 組織: <20 mg
細胞: <5 バツ 106 |
| 収率 | 2-50メートルg |
| 溶出量 | ≥30μL |
| 実行あたりの時間 | ≤25 minutes |
原理
- Purification Method: Silica column purification is employed in this product for effective nucleic acid extraction.
- Paraffin Removal: Buffer DPS is utilized to remove paraffin from the samples prior to the purification process.
- Sample Lysis and Digestion: The sample lysis process, coupled with proteinase K digestion, takes only 15 分, ensuring rapid processing.
- Incubation Conditions: Following lysis, samples are subjected to a 15-minute incubation at 80°C to facilitate optimal nucleic acid release.
- Nucleic Acid Adsorption: Transfer of the lysed samples to an adsorption column allows RNA to adsorb onto the membrane selectively, while proteins remain unadsorbed and are removed through filtration.
- Washing Step: Proteins and other impurities are washed away from the membrane to enhance the purity of the extracted RNA.
- 溶出: ついに, RNA is eluted from the membrane using a low-salt buffer, ensuring efficient recovery of high-quality RNA.
利点
- Efficient DNA removal – One-step RNA抽出 can effectively remove genomic DNA
- 高品質 – one-step RNA extraction reagent combined with silica gel column can obtain the highest concentration
- 速い – the whole extraction only takes 15-25 分
- Nontoxic – no toxic phenol-chloroform extraction is required in the extraction
キット内容
| コンテンツ | IVD4121 |
| 浄化時間 | 50 準備 |
| HiPure DNA Mini Column Ⅱ | 50 |
| Hipure RNAミニ柱 | 50 |
| 2ml 採取管 | 150 |
| プロテイナーゼK | 24 mg |
| プロテアーゼ溶解バッファー | 1.8 ミリリットル |
| DNase I | 600 μl |
| DNase Buffer | 6 ミリリットル |
| Buffer RTL | 40 ミリリットル |
| RNA Digestion Buffer | 15 ミリリットル |
| Buffer RWC* | 20 ミリリットル |
| バッファRW2* | 20 ミリリットル |
| Nuclease Free Water | 10 ミリリットル |
保管と安定性
- Proteinase K Storage: Store Proteinase K at 2–8°C upon arrival. 短期保管 (まで 8 週) 室温で (15–25℃) is acceptable without affecting its performance.
- DNase I Storage: Store DNase I at -20°C. 短期保管 (まで 1 週) 室温で (15–25℃) パフォーマンスには影響しません.
- Remaining Kit Components Storage: 残りのキットコンポーネントは室温で保存できます (15–25℃) 少なくとも安定したままです 18 この状況下で数か月.
レビュー
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