導入

• HiPure プラスミドDNA プラス 96 キット:
  • Enables up to 96 mini-preps to be performed simultaneously.
  • Process completed in less than 45 minutes using the Vacuum Manifold (Qiavac 96).
  • Provides a fast, simple, and cost-effective method for high-throughput plasmid DNA extraction in routine molecular biology laboratory applications.
• 主な特長:
  • Utilizes silica-membrane technology, eliminating cumbersome steps associated with loose resins or slurries.
  • Plasmid DNA purified with the kit is immediately ready for use.
  • Eliminates the need for phenol extraction and ethanol precipitation.
  • High-quality plasmid DNA is eluted in a small volume of Tris buffer or water.

詳細

仕様

原理

• HiPure Plasmid Procedure:
  • Based on alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt.
  • Utilizes a unique silica membrane that replaces glass or silica slurries for plasmid DNA minipreps.
• Procedure Steps:
  1. Preparation and Clearing of Bacterial Lysate:
     • The alkaline method is used to prepare and clear bacterial lysate.
  2. DNA Binding:
     • Supernatant transferred to a column to bind DNA to the silica membrane.
  3. Washing and Elution:
     • Proteins and other impurities washed away.
     • Nucleic acid was finally eluted with low-salt buffer (10mmトリス, pH9.0, 0.5mm EDTA).

利点

• 高純度 – 精製されたプラスミドは配列決定に直接使用できます, enzyme digestion PCR, 等.
• 速い – それだけが必要です 60 隔離が完了するまでに数分
• 高収量 – up to 1mg plasmid can be bound in one column
• 経済 – high cost-performance

キット内容

保管と安定性

キットのコンポーネントは乾燥した状態で室温で保管できます。 (15-25℃) 少なくとも一定期間は安定しています 18 この状況下で数か月. バッファー内に沈殿物が形成された場合, 37℃に温めて溶かす. After the addition of RNase A， バッファ P1 は次の期間安定しています。 6 2～8°Cで保管した場合、数か月.

購入ガイド