HiPure プラスミド ミニ キット

導入

• 速い, 単純, and Cost-Effective: Offers a rapid, easy-to-use, and economical method for プラスミドDNA miniprep suitable for routine molecular biology applications.
• Silica Membrane Technology: Utilizes silica membrane technology, eliminating the need for cumbersome steps associated with loose resins or slurries, streamlining the purification process.
• Immediate Usability: Purified plasmid DNA is immediately ready for downstream applications, saving time and effort.
• No Phenol Extraction or Ethanol Precipitation: Bypasses the need for phenol extraction and ethanol precipitation, simplifying the workflow and reducing hands-on time.
• High-Quality DNA: Yields high-quality plasmid DNA eluted in a small volume of Tris buffer or water, ensuring optimal performance in downstream applications.

仕様

原理

• Alkaline Lysis: Begins with the alkaline lysis of bacterial cells, which breaks down the cell membrane and releases plasmid DNA into the lysate.
• Silica-Based DNA Binding: The released DNA is adsorbed onto the silica membrane in the presence of high salt, facilitating efficient binding of DNA to the membrane surface.
• Three Basic Steps: The procedure involves three main steps: (1) Preparation and clearing of the bacterial lysate using an alkaline method, (2) Transfer of the cleared lysate to a column for DNA binding, そして (3) Washing to remove proteins and other impurities.
• Silica Membrane: Utilizes a unique silica membrane in the kit, which completely replaces traditional glass or silica slurries typically used in plasmid DNA minipreps.
• Elution with Low-Salt Buffer: After washing to remove impurities, nucleic acid, including plasmid DNA, is eluted from the silica membrane using a low-salt buffer containing 10mM Tris, pH 9.0, and 0.5mM EDTA.

利点

• 高純度 – 精製されたプラスミドは配列決定に直接使用できます, enzyme digestion PCR, 等.
• 速い – それだけが必要です 1 minute to obtain supernatant by optimized solution (10 minutes for other brands)
• 高収量 – up to 35µg plasmid can be bound in one column

キット内容

保管と安定性

• 保管条件: Kit components can be stored dry at room temperature (15-25℃) and remain stable for a minimum of 18 この状況下で数か月.
• Buffer Precipitates: In case of any precipitates forming in the buffers, warm them at 37°C to dissolve effectively.
• Buffer P1 Stability: After the addition of RNase A, Buffer P1 remains stable for up to 6 2～8°Cで保管した場合、数か月.

実験データ

購入ガイド