導入

The HiPure Liquid RNA & miRNA Kit integrates phenol/グアニジン-based sample lysis and silica-membrane purification of total RNA. MagZol LS Reagent, included in the kits, is a monophasic solution of phenol and guanidine thiocyanate designed to facilitate lysis of liquid sample and inhibit RNase. The high lysis efficiency of the reagent and the subsequent removal of contaminants by organic phase extraction enable the use of up to 0.25ml liquid sample per mini スピンカラム.

詳細

仕様

原理

0.25ml liquid samples are homogenized in 0.75ml MagZol LS Reagent. After the addition of chloroform, the homogenate is separated into aqueous and organic phases by centrifugation. The upper, aqueous phase is extracted, and ethanol is added to provide appropriate binding conditions. The sample is then applied to the spin column, where the total RNA (まで 100 μg) binds to the membrane and phenol and other contaminants are efficiently washed away. High-quality RNA is then eluted in 30–100µl of RNase-free water.

利点

• 高品質 – 高純度総RNAは、さまざまな敏感な下流のアプリケーションで直接使用できます
• Fast – isolation of several samples can be completed in 40 minutes by using the column purification method
• Safety – no phenol-chloroform extraction is required
• センシティブ – direct lysis of blood, プラズマ, and other samples without separation of leukocytes

キット内容

保管と安定性

MagZol LS Reagent should be stored at 2-8°C upon arrival. しかし, 短期保管 (まで 24 週) 室温で (15-25℃) 彼らのパフォーマンスには影響しない. 残りのキットコンポーネントは乾燥した状態で室温で保管できます。 (15-25℃) 少なくとも一定期間は安定しています 18 この状況下で数か月.