HiPure FFPE DNA キット

The HiPure FFPE Nucleic Acid Kit offers a fast and user-friendly solution for extracting DNA from formalin-fixed, パラフィン包埋 (FFPE) tissue samples.

• Target Samples: FFPE tissue and sections
• Simple and Rapid: DNA extraction can be completed in just 20 分 (excluding digestion time).
• Silica Gel Technology: Employs a reliable silica gel column purification method, eliminating the need for harsh organic solvents (phenol-chloroform) or alcohol precipitation steps.
• ダウンストリームアプリケーション: Purified DNA is suitable for various analyses like PCR (ポリメラーゼ連鎖反応), サザンブロット, and viral DNA detection.

利点:

• Streamlined Workflow: The silica gel column technology offers a faster and less hazardous alternative to traditional methods.
• High-Quality DNA: The extraction process ensures the isolation of DNA suitable for diverse downstream applications.
• Broad Applicability: The kit works effectively with various FFPE tissue samples.

This kit empowers researchers working in various fields, including oncology, pathology, および感染症, to efficiently extract DNA from archived FFPE samples for further analysis.

仕様

特徴	仕様
主な機能	Isolation of total DNA from FFPE tissue samples
アプリケーション	PCR, southern blot viral DNA detection, 等.
精製方法	ミニ スピンカラム
精製技術	シリカ技術
加工方法	マニュアル (遠心分離または真空)
サンプルの種類	Formalin-fixed, パラフィン包埋 (FFPE) tissue and section samples
サンプル量	<20mg
溶出量	<20μl
実行あたりの時間	<スパン “=”” スタイル=”概要: 0ピクセル; ボックスのサイジング: ボーダーボックス; フォントサイズ: 16ピクセル;”>≤20 minutes
カラムあたりの液体搬送量	4ミリリットル
カラムの結合収量	100μg

原理

HiPure FFPE Nucleic Acid Kit, designed for rapid and efficient extraction of both DNA and RNA from formalin-fixed, パラフィン包埋 (FFPE) tissue samples:

主な特長:

• Silica Gel Column Technology: Utilizes a reliable and streamlined approach, eliminating the need for harsh chemicals like phenol-chloroform.
• Fast Processing: Extracts DNA/RNA in just 20 分, excluding digestion time.
• Sample Compatibility: Works effectively with various FFPE tissue sections.

抽出プロセス:

1. Deparaffinization: Xylene removes paraffin wax, a common component used to preserve tissues.
2. 溶解と消化: A special solution (溶解物) と酵素 (プロテアーゼ) break down cells and proteins, releasing nucleic acids (DNA and RNA) into the solution.
3. De-Crosslinking: Heating at 90°C reverses the effects of formalin fixation, allowing easier access to nucleic acids.
4. Binding to Silica Membrane: After adjusting conditions with ethanol, the sample is transferred to a column. DNA/RNA binds to the silica membrane, while proteins pass through.
5. 洗浄: Buffers remove impurities like proteins and salts from the captured nucleic acids.
6. 溶出: A low-salt buffer elutes the purified DNA/RNA, making it ready for downstream applications.

アプリケーション:

• PCR (ポリメラーゼ連鎖反応)
• サザンブロット
• Viral nucleic acid detection
• Other analyses requiring high-quality DNA or RNA

利点

The HiPure FFPE Nucleic Acid Kit offers a superior solution for extracting DNA and RNA from formalin-fixed, パラフィン包埋 (FFPE) tissue samples.

Safety:

• No Xylene Contact: Eliminates the need for hazardous xylene, minimizing exposure to harmful chemicals.

スピード:

• Fast Processing: Extracts DNA/RNA in just 20 分 (excluding digestion time), allowing you to process multiple samples within 2 時間.
• No Overnight Incubation: Streamlines the workflow by eliminating the need for lengthy overnight incubation steps.

高効率:

• Enhanced PCR Sensitivity: Effectively removes formaldehyde modifications from DNA, significantly improving the sensitivity of downstream PCR (ポリメラーゼ連鎖反応) analyses.

キット内容

コンテンツ	D312602	D312603
浄化時間	50 準備	250 準備
HiPure DNA Mini Columns I	50	250
2ml 採取管	50	250
Buffer DPS	30 ミリリットル	150 ミリリットル
バッファATL	15 ミリリットル	60 ミリリットル
バッファAL	15 ミリリットル	60 ミリリットル
バッファGW1*	22 ミリリットル	88 ミリリットル
バッファgw2*	12 ミリリットル	50 ミリリットル
プロテイナーゼK	24 mg	120 mg
プロテアーゼ溶解バッファー	1.8 ミリリットル	10 ミリリットル
バッファAE	10 ミリリットル	30 ミリリットル

保管と安定性

• プロテイナーゼK: Keep it cool in the refrigerator (2-8℃) 到着時に最大の効果を得る.
• Short-Term Storage (まで 12 週): Proteinase K can last at room temperature (15-25℃) までのために 12 weeks without issues.
• Everything Else: Store other kit components at room temperature (15-25℃). They are stable for at least 18 この状況下で数か月.
• Important: If storing the entire kit cold, remember to warm up the buffers before use. All buffers should be at room temperature for optimal results.

実験データ