HiPure Circulating DNA Mini Kit, designed to efficiently purify short, cell-free DNA (cfDNA) molecules from human biological fluids:

• ターゲット:
  • Tumor-derived cfDNA fragments and mRNAs in blood
  • Fetal nucleic acids in maternal blood
• Sample Compatibility:
  • Human plasma
  • Human serum
  • Human urine
  • Samples can be fresh or frozen (once only)
• Focus on Short DNA: The kit isolates short nucleic acids (<1000血圧) commonly found in biofluids.
• Efficient Purification: The protocol allows for effective recovery of cfDNA from various biofluids.
• Ready for Downstream Use: Purified cfDNA is suitable for diverse applications, 含む:
  • PCR and other amplification reactions
  • 次世代シーケンス (NGS) analyses

利点:

• Optimized for Short cfDNA: Specifically designed to capture short DNA fragments relevant for biomarker discovery and liquid biopsies.
• Broad Sample Compatibility: Works effectively with various human biofluids, offering flexibility in sample selection.
• Streamlined Workflow: The miniaturized kit format allows for faster processing compared to larger kits.

This kit empowers researchers to study circulating DNA in various fields, including oncology, non-invasive prenatal testing (スキュー), and liquid biopsy applications.

仕様

原理

This product utilizes silica gel columns for a rapid and reliable DNA精製 process.

1. 溶解と消化: The sample undergoes treatment with a lysate solution and protease. This breaks open cells and degrades proteins, DNAを溶液に放出します.

2. Binding to Silica Membrane: The lysate containing the DNA is transferred to an adsorption plate or filtration column. The silica membrane in these tools selectively binds DNA molecules due to favorable interactions, while proteins and other impurities pass through.

3. 洗浄: Wash buffers are passed through the column, removing unbound proteins and cellular debris that didn’t adhere to the silica.

4. 溶出: A low-salt buffer (通常 10 mM トリス, pH 8.0) is used to elute the purified DNA from the silica membrane. This disrupts the interaction between DNA and the silica, allowing the collection of purified DNA in a separate tube.

This method provides a valuable tool for researchers working in various fields that require purified DNA for analysis, such as PCR, サザンブロット, および次世代シーケンス.

利点

• 高収量 – most optimal process, free DNA (>50血圧) can be obtained to the maximum extent
• 高濃度 – low elution volume, ensuring high nucleic acid concentration
• 高純度 – low alcohol binding method, completely removing inhibitor and protein pollution
• 高い回復力 – DNA can be recovered at the level of PG by silica gel column purification

キット内容

保管と安定性

Here’s a quick guide on storing your DNA extraction kit to ensure it functions at its best:

理想的なストレージ:

• Store Proteinase K and Carrier RNA in the refrigerator (2-8℃) 到着時に最大の効果を得る.

許容可能な短期ストレージ:

• Proteinase K and Carrier RNA can be kept at room temperature (15-25℃) までのために 12 パフォーマンスに影響を与えることなく週.

残りのコンポーネントの保存:

• All other kit components should be stored dry at room temperature (15-25℃).
• 少なくとも安定したままになります 18 この状況下で数か月.

重要なメモ:

• キット全体を2〜8°Cで保存する場合, 使用する前にバッファーを再溶解することを忘れないでください.
• 最適な結果を得るために使用する前に、すべてのバッファーが室温に達することを常に確認してください.

By following these storage recommendations, you can guarantee your DNA extraction kit is always ready for use and delivers the best possible results.