Kit HiPure RNA totale 50T- IVD4121

introduzione

This product offers a fast and reliable method to isolate RNA, including miRNA, from a wide range of samples:

• Tessuto
• Cellule
• Sangue
• Other clinical samples

The extracted RNA is suitable for various downstream applications, Compreso:

• RT-PCR (Reverse Transcription PCR)
• Quantitative RT-PCR (qRT-PCR)
• Viral RNA detection
• Other RNA-based analyses

This highlights the product’s versatility and compatibility with various research needs in gene expression analysis and RNA detection.

Specifiche

Caratteristiche	Specifiche
Funzioni principali	Isolation of total RNA（miRNA）from tissue, and cell using two columns and DNase plus reagent
Applicazioni	RT-PCR, Sintesi di cDNA, second-generation sequencing
Prodotti	RNA, miRNA
Metodo di purificazione	Mini colonna rotante
Tecnologia di purificazione	Tecnologia della silice
Metodo di processo	Manuale (centrifugazione o vuoto)
Tipo di campione	Clinical tissues, cellule, linfociti
Importo del campione	Tessuto: <20 mg Cellule: <5 X 106
Prodotto	2-50MG
Volume di eluizione	≥30μl
Tempo per corsa	≤25 minutes

Principio

• Purification Method: Silica column purification is employed in this product for effective nucleic acid extraction.
• Paraffin Removal: Buffer DPS is utilized to remove paraffin from the samples prior to the purification process.
• Lisi e digestione del campione: The sample lysis process, coupled with proteinase K digestion, takes only 15 minuti, ensuring rapid processing.
• Incubation Conditions: Following lysis, samples are subjected to a 15-minute incubation at 80°C to facilitate optimal nucleic acid release.
• Nucleic Acid Adsorption: Transfer of the lysed samples to an adsorption column allows RNA to adsorb onto the membrane selectively, while proteins remain unadsorbed and are removed through filtration.
• Washing Step: Proteins and other impurities are washed away from the membrane to enhance the purity of the extracted RNA.
• Eluizione: Finalmente, RNA is eluted from the membrane using a low-salt buffer, ensuring efficient recovery of high-quality RNA.

Vantaggi

• Efficient DNA removal – One-step Estrazione dell'RNA can effectively remove genomic DNA
• Alta qualità – one-step RNA extraction reagent combined with silica gel column can obtain the highest concentration
• Veloce – the whole extraction only takes 15-25 minuti
• Nontoxic – no toxic phenol-chloroform extraction is required in the extraction

Contenuto del kit

Contenuti	IVD4121
Tempi di purificazione	50 Preparativi
HiPure DNA Mini Column Ⅱ	50
HiPure RNA Mini Columns	50
2ml Provette di raccolta	150
Proteinasi K	24 mg
Tampone di dissoluzione della proteasi	1.8 ml
DNasi I	600 ml
Tampone dnase	6 ml
Buffer RTL	40 ml
RNA Digestion Buffer	15 ml
Buffer RWC*	20 ml
Buffer RW2*	20 ml
Nuclease Free Water	10 ml

Conservazione e stabilità

• Proteinase K Storage: Store Proteinase K at 2–8°C upon arrival. Short-term storage (fino a 8 settimane) a temperatura ambiente (15–25°C) is acceptable without affecting its performance.
• DNase I Storage: Store DNase I at -20°C. Short-term storage (fino a 1 settimana) a temperatura ambiente (15–25°C) does not affect its performance.
• Remaining Kit Components Storage: I restanti componenti del kit possono essere conservati a temperatura ambiente (15–25°C) and remain stable for at least 18 mesi in queste condizioni.