This product is suitable for extracting total RNA from 50-150mg conventional plantor fungal samples as well as samples rich in polyphenolic and polysaccharides. This kit is based on silica gel column purification technology, and the extraction process does not require the use of toxic phenol chloroform extraction. The entire extraction process only takes 20-30 minuti. This kit adopts DNA filtration technology, which can efficiently filter and remove DNA. L'RNA ottenuto può essere utilizzato direttamente per esperimenti come la RT-PCR, Northern Blot, poly A+ purification, protezione degli acidi nucleici, e traduzione in vitro.
Dettagli
Contenuto del kit
| Contenuti | R415002 | D415003 |
| Tempi di purificazione | 50 Preparativi | 250 Preparativi |
| HiPure RNA Mini Columns | 50 | 250 |
| gDNA Filter Columns | 50 | 250 |
| 2ml Provette di raccolta | 100 | 500 |
| TCEP (1M) | 0.29 G | 5 X 0.29 G |
| Buffer EP | 1.0 ml | 5.0 ml |
| Buffer PSL | 50 ml | 250 ml |
| Buffer RW1 | 50 ml | 250 ml |
| Buffer RW2* | 20 ml | 2 X 50 ml |
| Acqua libera da RNasi | 10 ml | 30 ml |
Conservazione e stabilità
Except TCEP, the kit components can be stored dry at room temperature (15–25°C) e sono stabili almeno per 18 mesi in queste condizioni. After receiving the product, it is recommended to store TCEP (dry powder) at -20-8°C. At low temperatures, Buffer PSL may form precipitates, dissolve it completely by 55°C water bath.
Recensioni
Non ci sono ancora recensioni.