Kit HiPure FFPE DNA

The HiPure FFPE Nucleic Acid Kit offers a fast and user-friendly solution for extracting DNA from formalin-fixed, paraffin-embedded (Ffpe) tissue samples.

• Target Samples: FFPE tissue and sections
• Simple and Rapid: DNA extraction can be completed in just 20 minuti (excluding digestion time).
• Silica Gel Technology: Employs a reliable silica gel column purification method, eliminating the need for harsh organic solvents (phenol-chloroform) or alcohol precipitation steps.
• Downstream Applications: Purified DNA is suitable for various analyses like PCR (Reazione a catena della polimerasi), Southernblotting, and viral DNA detection.

Benefici:

• Streamlined Workflow: The silica gel column technology offers a faster and less hazardous alternative to traditional methods.
• DNA di alta qualità: The extraction process ensures the isolation of DNA suitable for diverse downstream applications.
• Broad Applicability: The kit works effectively with various FFPE tissue samples.

This kit empowers researchers working in various fields, including oncology, pathology, and infectious diseases, to efficiently extract DNA from archived FFPE samples for further analysis.

Specifiche

Caratteristiche	Specifiche
Funzioni principali	Isolation of total DNA from FFPE tissue samples
Applicazioni	PCR, southern blot viral DNA detection, eccetera.
Metodo di purificazione	Mini colonna rotante
Tecnologia di purificazione	Tecnologia della silice
Metodo di processo	Manuale (centrifugazione o vuoto)
Tipo di campione	Formalin-fixed, paraffin-embedded (Ffpe) tissue and section samples
Importo del campione	<20mg
Volume di eluizione	<20ml
Tempo per corsa	<arco “=”” stile=”contorno: 0px; dimensionamento della scatola: scatola di confine; dimensione del font: 16px;”>≤20 minutes
Volume di trasporto del liquido per colonna	4ml
Resa legante della colonna	100μg

Principio

HiPure FFPE Nucleic Acid Kit, designed for rapid and efficient extraction of both DNA and RNA from formalin-fixed, paraffin-embedded (Ffpe) tissue samples:

Caratteristiche principali:

• Silica Gel Column Technology: Utilizes a reliable and streamlined approach, eliminating the need for harsh chemicals like phenol-chloroform.
• Fast Processing: Extracts DNA/RNA in just 20 minuti, excluding digestion time.
• Sample Compatibility: Works effectively with various FFPE tissue sections.

Processo di estrazione:

1. Deparaffinization: Xylene removes paraffin wax, a common component used to preserve tissues.
2. Lysis and Digestion: A special solution (lysate) and an enzyme (protease) break down cells and proteins, releasing nucleic acids (DNA and RNA) into the solution.
3. De-Crosslinking: Heating at 90°C reverses the effects of formalin fixation, allowing easier access to nucleic acids.
4. Binding to Silica Membrane: After adjusting conditions with ethanol, the sample is transferred to a column. DNA/RNA binds to the silica membrane, while proteins pass through.
5. Washing: Buffers remove impurities like proteins and salts from the captured nucleic acids.
6. Elution: A low-salt buffer elutes the purified DNA/RNA, making it ready for downstream applications.

Applicazioni:

• PCR (Reazione a catena della polimerasi)
• Southernblotting
• Viral nucleic acid detection
• Other analyses requiring high-quality DNA or RNA

Vantaggi

The HiPure FFPE Nucleic Acid Kit offers a superior solution for extracting DNA and RNA from formalin-fixed, paraffin-embedded (Ffpe) tissue samples.

Safety:

• No Xylene Contact: Eliminates the need for hazardous xylene, minimizing exposure to harmful chemicals.

Velocità:

• Fast Processing: Extracts DNA/RNA in just 20 minuti (excluding digestion time), allowing you to process multiple samples within 2 ore.
• No Overnight Incubation: Streamlines the workflow by eliminating the need for lengthy overnight incubation steps.

Alta efficienza:

• Enhanced PCR Sensitivity: Effectively removes formaldehyde modifications from DNA, significantly improving the sensitivity of downstream PCR (Reazione a catena della polimerasi) analyses.

Contenuto del kit

Contenuti	D312602	D312603
Tempi di purificazione	50 Preparativi	250 Preparativi
HiPure DNA Mini Columns I	50	250
2ml Provette di raccolta	50	250
Buffer DPS	30 ml	150 ml
ATL tampone	15 ml	60 ml
Buffer al	15 ml	60 ml
Buffer GW1*	22 ml	88 ml
Buffer GW2*	12 ml	50 ml
Proteinasi K	24 mg	120 mg
Tampone di dissoluzione della proteasi	1.8 ml	10 ml
Buffer ae	10 ml	30 ml

Conservazione e stabilità

• Proteinasi K: Keep it cool in the refrigerator (2-8°C) upon arrival for maximum effectiveness.
• Short-Term Storage (fino a 12 settimane): Proteinase K can last at room temperature (15-25°C) for up to 12 weeks without issues.
• Everything Else: Store other kit components at room temperature (15-25°C). Sono stabili per almeno 18 mesi in queste condizioni.
• Important: Se si conserva l'intero kit freddo, remember to warm up the buffers before use. All buffers should be at room temperature for optimal results.

Dati dell'esperimento