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Perkenalan
Poly A, polyadenylate, is a mixture of 100-10000 polyadenylates, which is polymerized by polynucleotide phosphorylasein vitro. In vivo, poly (A) is added to the 3-terminal of mRNA by enzyme to improve the stability of mRNA. Dalam penerapan ekstraksi asam nukleat, adding poly A to the lysate or binding solution can improve the yield of DNA and RNA. The mechanism of poly A improving the yield of nucleic acid is as follows:
- Saturated contact with the surface adsorption of articles. Most polypropylene articles have static electricity on the surface, which will adsorb nucleic acids. Carrier RNA can saturate these adsorption effects and reduce the loss of target nucleic acids.
- Inactivate trace nucleases: There are various nucleases in biological samples and environment. Poly A can inactivate trace nucleases in the extraction or preservation steps to improve the yield and stability of target nucleic acids.
- Coprecipitation: In the nucleic acid purification step of alcohol mediated precipitation or binding, poly A can coprecipitate with the target nucleic acid or form polymerparticles to improve the recovery.
Detail
Spesifikasi
| Fitur | Spesifikasi |
| Penampilan | White lyophilized powder |
| Kemurnian | 99% |
| Berat molekul | 700-3500 KDa |
| Kondisi transportasi | Suhu kamar |
| Kondisi penyimpanan | -20-8°C, dry storage, long-term storage should be placed at -20°C. |
| Usage method | Take an appropriate amount of lyophilized powder, add DEPC treated water or guanidin salt solution to dissolve it into 0.1-1μg/μl, and then subpack it and store it at -20°C. |
| Aplikasi | 1. Virus DNA/Ekstraksi RNA: adding 1-5μg Carrier RNA to the lysate can improve the yield of RNA/DNA, stabilize the target nucleic acid and avoid the degradation of the purified nucleic acid during storage.2. In the micro DNA/RNA extraction by column membrane method (<1μg), adding carrier RNA to 1-5μg is conducive to improve the yield of nucleic acid.
3. In the alcohol mediated nucleic acid precipitation and concentration step, the addition of 1-2μg carrier RNA is helpful to improve the recovery of short segment RNA. 4. In the quantitative probe PCR reaction solution, adding 10-100ng carrier RNA to the reaction solution is helpful to improve the sensitivity and reduce the CT value. |
Keuntungan
- No pollution – no DNase and RNase pollution
- Kemurnian tinggi – no PCR/ RT-PCR inhibition
- High degree of polymerization – 80% polymer is 100-2000nt
- No human – source DNA, no novel coronavirus pollution
Meminta informasi
| Isi | C12110 | C12111 | C12112 |
| Poly A, Liofilisasi | 310μg/Tube | 2g/Bottle | 100-1000μg/Tube, customized |
Ulasan
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