Perkenalan
This product is specially designed for stool Ekstraksi RNA. The kit is suitable for extracting high-purity microbial or host cell RNA from ≤ 0.15g stool samples. The purified RNA can be directly used in RT-PCR and Northern hybridization.
Detail
Prinsip
The Kit combines the speed and efficiency of silica-based technology with the convenient handling of magnetic particles for purification of total RNA. Samples are lysed and RNA is purified from lysates in one step through its binding to the silica surface of the particles in the presence of a chaotropic salt. The particles are separated from the lysates using a magnet and DNA is removed by treatment with RNase-free DNase. The magnetic particles are efficiently washed, and RNA is eluted in RNase-free water.
Isi Paket
| Isi | R662601 | R662602 | R662603 |
| Waktu Pemurnian | 48 Persiapan | 96 Persiapan | 5 X 96 Persiapan |
| Partikel RNA MagPure | 1.7 ml | 4.0 ml | 18 ml |
| DNase I | 600 μl | 2 X 600 μl | 10 X 600 μl |
| Penyangga DNAse | 30 ml | 40 ml | 200 ml |
| Penyangga SPL | 30 ml | 60 ml | 270 ml |
| Buffer PHC | 30 ml | 60 ml | 270 ml |
| Penyangga MCB* | 18 ml | 30 ml | 150 ml |
| Penyangga MW1* | 44 ml | 66 ml | 2 X 220 ml |
| Penyangga RW2* | 20 ml | 50 ml | 2 X 100 ml |
| Air Bebas RNase | 10 ml | 30 ml | 120 ml |
| 2tabung manik ML | 48 | 96 | 5 X 96 |
Penyimpanan dan Stabilitas
Partikel RNA MagPure harus disimpan pada suhu 2–8°C pada saat kedatangan. DNase I sebaiknya disimpan pada suhu -20°C. Namun, penyimpanan jangka pendek (DNase aku sampai 1 minggu, Partikel RNA MagPure hingga 8 minggu) pada suhu kamar (15–25°C) tidak mempengaruhi kinerja mereka. Komponen kit lainnya dapat disimpan pada suhu kamar (15–25°C) dan setidaknya stabil 18 months under these
Ulasan
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