A highly efficient, easily automated agarose gel or PCR purification system that delivers superior quality DNA with no salt carryover. Requiring no centrifugation or filtration. The Kit can be easily used in manual and automated 96 or 384-well formats.
Detail
Spesifikasi
| Fitur | Spesifikasi |
| Fungsi utama | Recover DNA from agarose Gel by magnetic bead |
| Aplikasi | PCR, NGS, chip analysis, dll.. |
| Teknologi pemurnian | Teknologi manik-manik magnetik |
| Metode proses | Manual atau otomatis |
| Jenis sampel | Agarose Gel, PCR products, enzymatic reaction solution |
| Jumlah sampel | Agarose Gel: appropriate amount, PCR products: ≤50µl |
| Recovery | 80% |
| Volume elusi | ≥30μl |
| Waktu per lari | ≤30 menit |
Prinsip
The Kit method contains magnetic particles in an optimized binding buffer to selectively bind DNA fragments (>100bp) and larger to paramagnetic beads. Excess primers, nucleotides, salts, and enzymes can be removed using a simple washing procedure.
Keuntungan
- High recovery efficiency – hingga 80% DNA recovery
- General – recover DNA from gel or enzyme-driven reaction solutions such as PCR
Isi Paket
| Isi | D500101 | D500102 | D500103 |
| Waktu Pemurnian | 50 Persiapan | 500 Persiapan | 5000 Persiapan |
| Buffer GDP | 30 ml | 250 ml | 3 X 900 ml |
| Partikel MagPure | 1.6 ml | 15 ml | 3 X 50 ml |
| Buffer DW1 | 20 ml | 180 ml | 2 X 800 ml |
| Penyangga Elusi(10mmTris, pH8.5) | 10 ml | 60 ml | 500 ml |
Penyimpanan dan Stabilitas
Partikel MagPure harus disimpan pada suhu 2-8°C pada saat kedatangan. Namun, penyimpanan jangka pendek (hingga 24 minggu) pada suhu kamar (15-25°C) tidak mempengaruhi kinerja mereka. Komponen kit lainnya dapat disimpan pada suhu kamar (15-25°C) dan setidaknya stabil 18 bulan dalam kondisi ini.
Data Eksperimen
Ulasan
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