HiPure Plasmid EF Mini Kit

Perkenalan

HiPure Plasmid EF Mini Kit Overview:

1. Technology Integration:
   • Combines HiPure technology with Magen’s innovative Endotoxin Removal Technology (ETR) to ensure high-quality plasmid pemurnian DNA.
2. Endotoxin Management:
   • Features a specially formulated buffer designed to prevent endotoxins from binding to the HiPure matrix, significantly reducing endotoxin levels in the final DNA product.
3. Application Suitability:
   • Provides plasmid DNA ideal for eukaryotic transfection and in vitro experiments, especially where low endotoxin levels are critical.
4. Impact of Endotoxin Contamination:
   • Endotoxin contamination can drastically lower transfection efficiencies, particularly in endotoxin-sensitive cell lines.
5. Clinical Relevance:
   • In contexts such as gene therapy, controlling endotoxin levels is crucial due to the potential for serious adverse effects like fever, endotoxin shock syndrome, and negative impacts on in vitro transfection into immune cells.

Detail

Spesifikasi

Prinsip

Prosedur HiPure Plasmid didasarkan pada lisis basa sel bakteri yang diikuti dengan adsorpsi DNA ke silika dengan adanya garam tinggi.. Membran silika unik yang digunakan dalam kit ini sepenuhnya menggantikan kaca atau bubur silika untuk miniprep DNA plasmid. Prosedurnya terdiri dari 3 langkah-langkah dasar: Persiapan dan pembersihan lisat bakteri dengan metode basa，then transfer the supernatant to column to bind DNA. Setelah mencuci protein dan kotoran lainnya, asam nukleat akhirnya dielusi dengan buffer rendah garam (10mm Tris, pH9.0, 0.5mm EDTA).

Keuntungan

• Kemurnian tinggi – Plasmid yang dimurnikan dapat langsung digunakan dalam pengurutan, enzyme digestion and PCR, dll..
• Cepat – silica gel column purification is much faster than ion exchange
• Hasil tinggi – up to 70μg plasmid can be binded in one column
• Low endotoxin content – the obtained plasmid can be directly used in cell transfection and animal injection

Isi Paket

Penyimpanan dan Stabilitas

Storage Instructions:

1. General Storage:
   • Kit components can be stored dry at room temperature (15–25°C).
   • Setidaknya stabil 18 bulan dalam kondisi ini.
2. Handling Precipitates:
   • Jika ada endapan yang terbentuk di buffer, warm at 37°C to dissolve.
3. Specific Storage for Buffer P1 with RNase A:
   • After addition of RNase A, Buffer P1 stabil untuk 6 months when stored at 2–8°C.

Panduan Pembelian