Perkenalan
Hipure Microbial RNA kit is suitable for extracting high-purity total RNA from bacterial/yeast culture medium. This kit combines high-efficiency Magzol Reagent (one-step extraction reagent) and silicagel column purification technology to complete the extraction of high-purity total RNA in only 40 menit. RNA yang didapat bisa langsung digunakan di RT-PCR, Northern blot, poly-A + pemurnian, Perlindungan asam nukleat dan terjemahan in vitro.
Detail
Spesifikasi
| Fitur | Spesifikasi |
| Fungsi utama | Isolation total RNA from bacteria, yeast cells |
| Aplikasi | RT-PCR, Northern blot, poly-A + pemurnian, Perlindungan asam nukleat dan terjemahan in vitro |
| Metode pemurnian | Mini kolom putar |
| Teknologi pemurnian | Teknologi silika |
| Metode proses | petunjuk (sentrifugasi atau vakum) |
| Jenis sampel | Bakteri, yeast cells |
| Jumlah sampel | Bakteri: <10^9；Yeast cells：<2 x10^7 |
| Volume elusi | ≥30μl |
| Waktu per lari | ≤40 minutes |
| Volume pembawa cairan per kolom | 800μl |
| Hasil pengikatan kolom | 100mg |
Prinsip
Hipure silica gel column is based on glass fiber filter membrane with high binding force. Under the condition of a high concentration of ionizing agent (seperti guanidin hydrochloride or guanidine isothiocyanate), the filter membrane can adsorb nucleic acid through hydrogen bond and electrostatic, while protein and other impurities are not adsorbed and removed. The filter membrane adsorbed with nucleic acid is washed to remove the residual protein and salt. Akhirnya, the nucleic acid adsorbed on the filter membrane can be washed out with low salt buffer (such as buffer TE) or water. The obtained nucleic acid has high purity and can be directly used in various downstream experiments.
Keuntungan
- Cepat – beberapa sampel dapat diekstraksi 30 menit
- Kemurnian tinggi – the purified RNA can be directly used in various downstream applications
- Pemulihan tinggi – RNA dapat dipulihkan pada tingkat PG
- Pengulangan yang baik – silica gel column purification technology can obtain ideal results every time
- Broad spectrum – it can deal with various bacteria, including Gram-positive bacteria that are difficult to be lysed
- Komponen yang cukup – the kit contains carried wall-breaking enzymes and glass beads
Isi Paket
| Isi | R418202 | R418203 |
| Waktu Pemurnian | 50 Persiapan | 250 Persiapan |
| Kolom Mini RNA HiPure | 50 | 250 |
| 2ml Tabung Koleksi | 50 | 250 |
| Manik -manik kaca (0.1-0.6mm) | 30 G | 150 G |
| DNase I | 600 μl | 5 X 600 μl |
| Penyangga DNAse | 6 ml | 30 ml |
| Buffer Larut Protease | 1.8 ml | 15 ml |
| Penyangga ATL | 50 ml | 200 ml |
| Buffer PHC | 50 ml | 200 ml |
| Buffer GXP2* | 20 ml | 100 ml |
| Buffer RW1 | 50 ml | 250 ml |
| Penyangga RW2* | 20 ml | 2 X 50 ml |
| Air Bebas RNase | 10 ml | 30 ml |
Penyimpanan dan Stabilitas
Buffer PHC should be stored at 2–8°C upon arrival. DNase I sebaiknya disimpan pada suhu -20°C. Namun, penyimpanan jangka pendek (DNase aku sampai 1 minggu, Buffer PHC up to 12 minggu) pada suhu kamar(15–25°C) tidak mempengaruhi kinerja mereka. The remaining kit components can be stored at roomtemperature (15–25°C) dan setidaknya stabil 18 bulan dalam kondisi ini.
Ulasan
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