Perkenalan
The Kit is specially designed for simultaneous purification of genomic DNA and total RNA from formalin-fixed, parafin-embedded (Ffpe) tissue sections. DNA/RNA yang dimurnikan digunakan untuk RT-PCR dan deteksi PCR.
Detail
Spesifikasi
| Fitur | Spesifikasi |
| Main FunctionsC | Co-isolation DNA and RNA from a single FFPE tissue sample |
| Aplikasi | RT-PCR, sintesis cDNA, PCR and second-generation sequencing, dll.. |
| Metode pemurnian | Mini kolom putar |
| Teknologi pemurnian | Teknologi silika |
| Metode proses | petunjuk (sentrifugasi atau vakum) |
| Jenis sampel | FFPE slice, FFPE embedded tissue |
| Jumlah sampel | No more than six 10µm sections of 150mm2 surface area or three 20µm sections of 150mm2 surface area. |
Prinsip
FFPE samples are incubated in an optimized lysis buffer, which results in the release of RNA and precipitation of DNA. Setelah sentrifugasi, the RNA-containing supernatant and DNA-containing pellet are then processed separately to purify RNA and DNA. For RNA purification, transfer RNA Lysate to an adsorption column and RNA is adsorbed on the membrane, sedangkan protein tidak diserap dan dihilangkan dengan filtrasi. Setelah mencuci protein dan kotoran lainnya, RNA akhirnya dielusi dengan buffer rendah garam. Untuk pemurnian DNA, transfer DNA Lysate to an adsorption column and DNA is adsorbed on the membrane, sedangkan protein tidak diserap dan dihilangkan dengan filtrasi. Setelah mencuci protein dan kotoran lainnya, DNA was finally eluted with low-salt buffer.
Keuntungan
- Kualitas tinggi – high purity total RNA / DNA can be directly used in various downstream applications
- Cepat – column method can complete the extraction of several samples in 30 menit
- Aman – Tidak diperlukan ekstraksi fenol kloroform
- Simultaneous extraction – simultaneously isolate DNA and RNA from one sample
Isi Paket
| Isi | IVD5116 |
| Waktu Pemurnian | 50 Persiapan |
| Kolom Mikro DNA HiPure | 50 |
| HiPure RNA Mini Column I | 50 |
| 2ml Tabung Koleksi | 150 |
| Proteinase K | 50 mg |
| Buffer Larut Protease | 5 ml |
| DPS buffer | 60 ml |
| Buffer FRL | 15 ml |
| Penyangga ATL | 15 ml |
| Buffer RLC | 15 ml |
| Penyangga AL | 15 ml |
| Buffer VHB | 44 ml |
| Buffer RW2 | 25 ml |
| Air Bebas RNase | 10 ml |
| Penyangga AE | 10 ml |
Penyimpanan dan Stabilitas
Proteinase K harus disimpan pada 2-8 ° C pada saat kedatangan. Namun, penyimpanan jangka pendek (hingga 12 minggu) pada suhu kamar (15-25°C) tidak mempengaruhi kinerja mereka. Komponen kit lainnya dapat disimpan pada suhu kamar (15-25°C) dan setidaknya stabil 18 bulan dalam kondisi ini.
Data Eksperimen
Ulasan
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