This kit provides a simple and fast solution for the extraction of circulating nuclear acid from serum, plasma, and other cell-free liquid samples. Circulating nucleic acid refers to the free extracellular nucleic acid produced by cell apoptosis, of which fragments are generally below 1KB. The kit is based on silica gel column purification technology, which is no need for toxic phenol chloroform extraction and time-consuming alcohol precipitation during the extraction. The obtained Circulating Nucleic Acid can be directly used for quantitative PCR, liquid or solid phase chip analysis, hibridisasi, dan deteksi SNP.
HiPure Circulating DNA/RNA Kit adopts a unique solution system and multiple layers of filter membranes with different pore sizes, which can efficiently process large volumes of serum and plasma samples and capture extremely small amounts of free nucleic acids.
Detail
Spesifikasi
| Fitur | Spesifikasi |
| Fungsi utama | Isolation both Circulating DNA/RNA (include miRNA) from 1-5 ml serum and plasma |
| Aplikasi | qPCR / RT-PCR, liquid or solid-phasechip analysis, hybridization and SNP detection |
| Metode pemurnian | Midi kolom putar |
| Teknologi pemurnian | Teknologi silika, DNA filtration technology |
| Metode proses | petunjuk (sentrifugasi atau vakum) |
| Jenis sampel | serum, plasma, and other cell-free liquid samples |
| Jumlah sampel | 1-5 ml |
| Volume elusi | ≥20μl |
| Waktu per lari | ≤100 minutes |
| Volume pembawa cairan per kolom | 4 ml |
| Hasil pengikatan kolom | 1 mg |
Prinsip
This kit is based on silica gel column technology. Serum or other liquid samples are lysed and digested in buffer CFL. After adding buffer CFP, the protein is removed by centrifugation to obtain the supernatant. Isopropanol is added to precipitate the total nucleic acid and transferred to the column for filtration. DNA / RNA is adsorbed on the membrane of the column, while the protein is not adsorbed and removed with the filtrate.The column is washed with buffer MGW1 to remove protein and other impurities, and then washed with buffer RW2 to remove salt. Akhirnya, DNA / RNA is eluted by low salt buffer. The eluted DNA / RNA can be directly used for quantitative PCR/ RT-PCR, analisis chip fase cair atau padat, hybridization and SNP detection.
Keuntungan
- Hasil tinggi – most optimized process to obtain maximum free RNA and small RNA
- Konsentrasi tinggi – volume elusi rendah (>20μl) to ensure nucleic acid concentration
- Kemurnian tinggi – low alcohol combination, completely remove inhibitor and protein pollution
- Pemulihan tinggi – silica gel column technology can recover nucleic acid molecules at the level of PG
- Large volume – 1-2ml serum and plasma samples can be processed at one time
Isi Paket
| Isi | R431602 | D431603 |
| Waktu Pemurnian | 50 Persiapan | 250 Persiapan |
| Kolom mikro Hipure RNA | 50 | 5 X 50 |
| HiPure Viral Midi Columns | 50 | 5 X 50 |
| 15 ml Tabung Koleksi | 50 | 5 X 50 |
| 2ml Tabung Koleksi | 50 | 5 X 50 |
| Buffer CFL | 150 ml | 2 X 375 ml |
| Buffer CFP | 30 ml | 150 ml |
| Buffer MGW1* | 100 ml | 2 X 250 ml |
| Penyangga RW2* | 2 X 50 ml | 5 X 100 ml |
| Air Bebas RNase | 10 ml | 50 ml |
Penyimpanan dan Stabilitas
Komponen kit dapat disimpan pada suhu kamar (15–25°C) dan setidaknya stabil 18 bulan dalam kondisi ini.
Ulasan
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