Tris HCl cas 1185-53-1

Uvod

• The products undergo rigorous screening and quality control to serve as the primary raw material for nucleic acid extraction.
• Buffer solutions are capable of resisting the impact of strong acids and bases, thereby maintaining a stable pH within the system. It’s crucial to uphold a pH range of 4.0 do 11.0 during nucleic acid extraction.
• Nucleic acids are prone to hydrolysis in acidic conditions but remain stable in neutral or weakly alkaline environments. Tris, or trimethylolaminomethane, functions as a pH buffer within the range of 7.0 do 9.0. Its role is to preserve the stability of nucleic acids post-sample cleavage, preventing degradation and enhancing concentration and purity.
• Tris-containing reagents cannot undergo treatment with DEPC, necessitating rigorous assurance of RNase absence during Tris preparation. Tipično, DEPC-treated water is employed for preparation to ensure RNase-free Tris of high purity, exceeding 99.99%.
• This product undergoes stringent testing for RNase and DNase activity, confirming its purity of over 99.99% and absence of DNase or RNase activity.

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