Solarbio Direct bilirubin (DBIL) Assay Kit 50T | 48S

Bilješka: Uzmite dva ili tri različita uzorka za predviđanje prije testa.

Operativna oprema: Spectrophotometer/Microplate reader

Catalog Number: BC5175

Veličina: 50T | 48S

Komponente:

Reagens i: 25 ml × 1. Store at 2-8℃.

Reagens II: 6 ml × 1. Store at 2-8℃.

Opis proizvoda：

Direct bilirubin (DBIL) is also called conjugated bilirubin. After indirect bilirubin enters the liver, it is combined with glucuronic acid by the action of glucuronosyltransferase in the liver. The increase of direct bilirubin is of great significance for clinical diagnosis of obstructive jaundice, hepatocellular jaundice, liver cancer, pancreatic head cancer, cholelithiasis, and cholangiocarcinoma. Direct bilirubin could be oxidized by sodium nitrite to form biliverdin, which has an absorbance of 450 NM. The content of direct bilirubin can be calculated by detecting the wavelength change at 450 NM.

Potrebni reagensi i oprema, ali nisu pruženi：

Spectrophotometer/Microplate reader, stolna centrifuga, constant temperature foster box/water bath, pipette, micro glass cuvette/96 well flat-bottom plate, led, and distilled water.

Postupak

A. Priprema uzorka:

Serum, plazma, or other liquid samples: Detect the sample directly. If the solution is turbid, measure centrifuging.

B.Odlučnost postupak：

1. Preheat the spectrophotometer/microplate reader for 30min, Podesite valnu duljinu na 450 NM, and set the spectrophotometer counter to zero with distilled
2. Odlučnost:

Reagens (μL)	Epruveta	Prazna cijev
Uzorak	8	–
Destilirana voda	–	8
Reagens i	192	192
Mix well. React at 37℃ for 5 minuta. Measure the absorbance at 450 NM, and record it as A1T, A1B;
Reagens II	48	48
Mix well. React at 37℃ for 5 minuta. Measure the absorbance at 450 NM, and record it as A2T, A2B. Calculate the ΔAT =A1T-A2T and the ΔAB =A1B-A2B. Blank tubes only need to be measured once or twice.

C.Calculations：

96 well flat-bottom plate

DBIL content (μmol/L) =1495.7×(ΔAT-ΔAB)-15.343

Micro glass cuvette

DBIL content (μmol/L) =1099.7×(ΔAT-ΔAB)-10.73

Bilješka:

1. Bilirubin decomposes easily in light. Avoid light during
2. If the ΔA is lower, it is recommended to increase the sample size before determination; If ΔA＞5, Preporučuje se razrijediti uzorak prije određivanja.

Examples:

1. Take mouse serum to follow the determination procedure to operate. Determination with 96 well flat-bottom plate, and calculate ΔAT=A1T-A2T=0.217-0.170=0.047, ΔAB=A1B-A2B=0.044-0.044=0. The calculated content is as follows:

DBIL content (μmol/L) =1495.7×(ΔAT-ΔAB)-15.343=54.95 μmol/L.

Povezani proizvodi

BC5180/BC5185 Total bilirubin (TBIL) Komplet za ispitivanje sadržaja