Uvod
This kit is suitable for high-throughput extraction of total RNA from cultured cells, cell suspensions, animal tissues, conventional fungi and plant tissues. The kit is based on superparamagnetic magnetic particle purification technology. The extraction process does not require the use of toxic phenolic chloroform extraction or time-consuming alcohol precipitation. Cijeli proces ekstrakcije traje samo 60 minuta. The obtained RNA can be directly used in RT-PCR, fluorescence quantification, second-generation sequencing and viral RNA detection and so on. The high-throughput extraction can be realized by 96 hole magnetic frame and 96 hole oscillator, various nucleic acid extractors (such as KingFisherFle, MagMix32, MagMix 96) or cooperating with the pipette workstation (Hamilton, Tican) for automatic extraction.
pojedinosti
Tehnički podaci
| Značajke | Tehnički podaci |
| Glavne funkcije | Isolation total RNA from tissue, cell |
| Prijave | RT-PCR, cDNA synthesis, second generation sequencing |
| Metoda pročišćavanja | Polydisperse magnetic beads |
| Tehnologija pročišćavanja | Tehnologija magnetskih kuglica |
| Procesna metoda | Ručno ili automatski |
| Adaptive instrument | Nucleic acid extractor, pipetting workstation |
| Vrsta uzorka | Tissues, stanice, lymphocytes and other clinical sample |
| Količina uzorka | Cells grown insuspension:≤1 x 107
Animal tissue: ≤20mg Plant tissue: ≤100mg Puna krv: ≤1.5ml |
| Prinos | 2-50μg |
Načelo
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and protease. After adding magnetic particles and binding solution, RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally RNA was eluted by Elution Buffer.
Prednosti
- Visoka čistoća – OD260 / 280 :1.9-2.0, OD 260 / 230 :1.5-2.0
- Economy – manje nego 50% of the price of Qiagen and other imported products
- Automatic – extraction without manual participation, saving time and effort
- Universal – suitable for various clinical samples
Sadržaj kompleta
| Sadržaj | IVD3020 |
| Vremena pročišćavanja | 200 Pripreme |
| MagPure RNA Particles | 7 ml |
| DNaza I | 4 x 600 µl |
| DNase pufer | 80 ml |
| RTL Lysis Buffer | 150 ml |
| Buffer MCB* | 75 ml |
| Buffer MW1* | 110 ml |
| Buffer MW2* | 50 ml |
| Voda bez RNaze | 60 ml |
Skladištenje i stabilnost
MagPure RNA Particles should be stored at 2–8°C upon arrival. DNazu I treba čuvati na -20°C. Međutim, kratkotrajno skladištenje (DNase I do 1 tjedni, MagPure RNA Particles up to 8 tjedni) at roomtemperature (15–25°C) ne utječe na njihovu izvedbu. Preostale komponente kita mogu se čuvati na sobnoj temperaturi (15–25°C) i stabilni su najmanje 18 mjeseci pod ovim uvjetima.
Recenzije
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