Uvod
The MagPure Plasmid purification system uses the paramagnetic bead technology for high-throughput preparation of high-copy or low-copy plazmidna DNK from E. coli cells. This kit also can be used with fosmid and BAC vector-based constructs. The system uses alkaline lysis followed by a MagPure purification to differentially bind plasmid DNA to paramagnetic beads. While the DNA is bound to the beads, contaminants can be rinsed away using a simple washing procedure. Because MagPure uses magnetic separation technology, the protocol does not require vacuum filtration. This makes kit extremely amenable to automation. Plasmid DNA purified with this system is most commonly used in Sanger Sequencing and PCR amplification.
pojedinosti
Tehnički podaci
| Značajke | Tehnički podaci |
| Glavne funkcije | Isolation up to 20µg plasmid DNA from 1-3ml bacterial culture |
| Prijave | Enzyme digestion, sekvenciranje, PCR and labeling, itd. |
| Tehnologija pročišćavanja | Tehnologija magnetskih kuglica |
| Procesna metoda | Ručno ili automatski |
| Vrsta uzorka | Konvencionalni plazmid, plasmid≤30KB |
| Količina uzorka | 1-3ml |
| Volumen eluacije | ≥50μl |
Načelo
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Lysozyme. DNK se oslobađa u lizat. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.
Prednosti
- Visoka čistoća – pročišćeni plazmid može se izravno koristiti u sekvenciranju, enzyme digestion and PCR, itd.
- Visok prinos – up to 20μg plasmid can be binded in one column
Sadržaj kompleta
| Sadržaj | P181102 | P181103 | P181104 |
| Vremena pročišćavanja | 100 Pripreme | 500 Pripreme | 5000 Pripreme |
| RNaza A | 10 mg | 50 mg | 2 x 250 mg |
| Buffer P1 | 30 ml | 150 ml | 2 x 750 ml |
| Buffer P2 | 30 ml | 150 ml | 2 x 750 ml |
| Buffer N3 | 30 ml | 150 ml | 2 x 750 ml |
| Buffer PW1 | 35 ml | 180 ml | 2 x 900 ml |
| MagPure Particle NB* | 2.2 ml | 11 ml | 2 x 60 ml |
Skladištenje i stabilnost
RNase A and MagPure Particle NB should be stored at 2-8°C upon arrival. Međutim, kratkotrajno skladištenje (do 24 tjedni) na sobnoj temperaturi (15-25°C) ne utječe na njegovu izvedbu. Preostale komponente kita mogu se čuvati na suhom na sobnoj temperaturi (15-25°C) i stabilni su najmanje 18 mjeseci pod ovim uvjetima. After addition of RNase A, Međuspremnik P1 je stabilan za 6 months when stored at 2-8°C.
Podaci o eksperimentu
Recenzije
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