Pregled proizvoda
| Značajke | Tehnički podaci |
| Concentration | 100 mg/ml |
| Izgled | Suspension of black particles |
| Surface functional group | Si-OH, Silanol |
| Dispersibility | Polydisperse Amorphous |
| Particle size | 1.5-5 µm |
| Uvjeti očuvanja | Sobna temperatura, valid for up to 2 godina.
It is recommended to store at 2-8°C to prevent microbial growth. |
| Magnetic response speed | 15-30 sekundi |
| Settling velocity | >5 minuta |
| High salt mediated binding | >2M gvanidin isothiocyanate, DNA recovery up to 80% |
| Alcohol mediated binding | 2M guanidine hydrochloride/isopropanol (30%), and the recovery of DNA / RNA was as high as 85% |
| PEG8000 mediated binding | The recovery of DNA/RNA was up to 85% |
| DNase/RNase | Nije otkriveno |
| DNA residue | <1 ppm |
| Preporučena prijava | Plasmid extraction, gel DNA recovery, genomic DNA extraction i RNA extraction. |
Načelo
Magnetic Separation:
- Magnetic beads with silica coating are used.
- After lysing the sample, DNA/RNA is released.
- Magnetic beads and binding solution are added.
- DNA/RNA binds to the surface of the beads.
- A magnet separates the bead-DNA/RNA complex from the solution (impurities).
Selective Binding:
- High salt-mediated binding (2-4M guanidine isothiocyanate): selectively recovers DNA molecules.
- Alcohol-mediated binding (guanidine salt and >25% alkohol): selectively recovers DNA/RNA molecules.
- Proteins and other impurities are not adsorbed to the beads.
Elution:
- The purified DNA/RNA complex is washed to remove contaminants.
- DNA/RNA is released from the beads using sterilized water or TE buffer.
Recenzije
Još nema recenzija.