Uvod
The Kit is specially designed for simultaneous purification of genomic DNA and total RNA from formalin-fixed, paraffin-embedded (FFPE) tissue sections. Purified DNA/RNA is suitable for use in applications such as real-time PCR and pyrosequencing.
pojedinosti
Tehnički podaci
| Značajke | Tehnički podaci |
| Glavne funkcije | Co-isolation DNA and RNA from FFPE tissue |
| Prijave | RT-PCR, cDNA synthesis, PCR and second-generation sequencing, itd. |
| Metoda pročišćavanja | Polydisperse silicon based magnetic beads (DNA)
Monodisperse carbonyl magnetic beads (RNA) |
| Tehnologija pročišćavanja | Tehnologija magnetskih kuglica |
| Procesna metoda | Ručno ili automatski |
| Adaptive instrument | Nucleic acid extractor and pipetting workstation |
| Vrsta uzorka | FFPE slice, FFPE puncture sample, embedded tissue |
| Količina uzorka | No more than six 10 µm sections of 150 mm2 surface area or three 20µm sections of 150 mm2 surface area |
| Prinos | DNA: 1 – 10 μg, RNA: 1 – 25 μg |
Načelo
The sample is lysed and digested under the action of lysate and protease. DNA/RNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, the supernatant contain RNA was collected and bind to MagBind Particles. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA/RNA was eluted by Elution Buffer.
Prednosti
- Visoka kvaliteta – high purity total RNA / DNA can be directly used in various downstream applications
- Sef – no phenol chloroform extraction required
- Simultaneous extraction – simultaneously isolate DNA and RNA from one sample
- Post digestion sorting – higher DNA and RNA yields
- High RNA yield – monodisperse carbonyl adsorption
Sadržaj kompleta
| Sadržaj | R632701 | R632702 | R632703 |
| Vremena pročišćavanja | 48 Pripreme | 96 Pripreme | 5 x 96 Pripreme |
| MagBind Particles | 1.1 ml | 2.5 ml | 11 ml |
| MagPure Particles N | 1.1 ml | 2.5 ml | 11 ml |
| Proteinaza K | 24 mg | 48 mg | 220 mg |
| Pufer za otapanje proteaze | 3 ml | 10 ml | 15 ml |
| Buffer DPS | 50 ml | 100 ml | 2 x 250 ml |
| Buffer ATL | 20 ml | 30 ml | 120 ml |
| Buffer BST1 | 20 ml | 40 ml | 200 ml |
| Buffer BXW1* | 44 ml | 110 ml | 3 x 110 ml |
| Voda bez RNaze | 15 ml | 30 ml | 120 ml |
Skladištenje i stabilnost
Proteinaza K, MagPure Particles N and MagBind Particles should be stored at 2–8°C upon arrival. Međutim, kratkotrajno skladištenje (do 12 tjedni) na sobnoj temperaturi (15–25°C) ne utječe na njihovu izvedbu. Preostale komponente kita mogu se čuvati na sobnoj temperaturi (15–25°C) and are stablefor at least 18 mjeseci pod ovim uvjetima.
Podaci o eksperimentu
Recenzije
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