Uvod
The Kit is specially designed for simultaneous purification of genomic DNA and total RNA from formalin-fixed, paraffin-embedded (FFPE) tissue sections. Purified DNA/RNA is suitable for use in applications such as real-time PCR and Pyrosequencing.
pojedinosti
Tehnički podaci
| Značajke | Tehnički podaci |
| Main FunctionsC | Co-isolation total RNA and DNA from FFPE tissue |
| Prijave | RT-PCR, cDNA synthesis, PCR and second-generation sequencing, itd. |
| Metoda pročišćavanja | Polydisperse magnetic beads |
| Tehnologija pročišćavanja | Tehnologija magnetskih kuglica |
| Procesna metoda | Ručno ili automatski |
| Adaptive instrument | Nucleic acid extractor and pipetting workstation |
| Vrsta uzorka | FFPE slice, FFPE puncture sample, embedded tissue |
| Količina uzorka | No more than six 10µm sections of 150 mm2 surface area or three 20µm sections of 150 mm2 surface area. |
Načelo
FFPE samples are incubated in an optimized lysis buffer, which results in the release of RNA and precipitation of DNA. After centrifugation, the RNA-containing supernatant and DNA-containing pellet are then processed separately to purify RNA and DNA. After adding magnetic particles and binding solution, DNA/RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA/RNA was eluted by RNase Free Water.
Prednosti
- Post digestion sorting – higher DNA and RNA yields
- Economy – the price is much lower than imported reagents
Sadržaj kompleta
| Sadržaj | IVD3026 |
| Vremena pročišćavanja | 200 Pripreme |
| MagBind Particles | 9.0 ml |
| Proteinaza K | 180 mg |
| Pufer za otapanje proteaze | 10 ml |
| Buffer DPS | 150 ml |
| Buffer FRL | 40 ml |
| Buffer ATL | 40 ml |
| Buffer AL | 80 ml |
| Buffer BXW1* | 110 ml |
| Voda bez RNaze | 30 ml |
Skladištenje i stabilnost
Proteinase K and MagBind Particles should be stored at 2-8°C upon arrival. Međutim, kratkotrajno skladištenje (do 24 tjedni) na sobnoj temperaturi (15-25°C) ne utječe na njihovu izvedbu. Preostale komponente kita mogu se čuvati na sobnoj temperaturi (15-25°C) i stabilni su najmanje 18 mjeseci pod ovim uvjetima.
Recenzije
Još nema recenzija.