Pregled proizvoda
- Offers fast and easy purification methods for total DNA.
- Ensures reliable PCR and Southern Blotting results.
- Capable of purifying various types of DNA, including genomic, viral, and mitochondrial.
- Can purify DNA from diverse sources: tissue, whole blood, plazma, serum, buffy coat, bone marrow, other body fluids, lymphocytes, and cultured cells.
Tehnički podaci
| Značajke | Tehnički podaci |
| Glavne funkcije | Isolation total DNA from blood, tissue, culture cells, swab, and blood spots using 96 plate |
| Prijave | PCR, Southern Bolt i otkrivanje virusa, itd |
| Metoda pročišćavanja | 96 bunar ploča |
| Tehnologija pročišćavanja | Silika tehnologija |
| Procesna metoda | Priručnik (centrifugiranje ili vakuum) |
| Vrsta uzorka | Krv, serum, plazma, milk, saliva, and other liquid samples and cultured cells |
| Količina uzorka | |
| Volumen eluacije | |
| Vrijeme po vožnji | |
| Nosivi volumen tekućine po stupcu | |
| Prinos vezivanja kolone |
Načelo
- Utilizes silica column purification method.
- Uzorak se lizira i probavlja lizatom i proteazom, releasing DNA into the lysate.
- Lysate is transferred to an adsorption column.
- Nukleinska kiselina se adsorbira na membrani, while protein is not adsorbed and is removed through filtration.
- Proteins and other impurities are washed away.
- Nucleic acid is finally eluted with low-salt buffer (10mm Tris, pH 9.0, 0.5mm EDTA).
Prednosti
- High-quality DNA – meets a variety of downstream applications, including PCR, qPCR, enzyme digestion, hybridization, itd.
- Visoka propusnost – 96 samples can be processed simultaneously
Sadržaj kompleta
| Sadržaj | D311701 | D311702 |
| Vremena pročišćavanja | 1 x 96 | 4 x 96 |
| HiPure gDNA Plate | 1 | 4 |
| 96 well Plate (2.2ml) | 1 | 4 |
| 1.6ml Collection Plate | 1 | 4 |
| 0.5ml Collection Plate | 1 | 4 |
| Silicon Seal Tape | 1 | 4 |
| Seal Film | 5 | 25 |
| Buffer ATL | 30 ml | 100 ml |
| Buffer AL | 30 ml | 100 ml |
| Međuspremnik DW1 | 60 ml | 250 ml |
| Međuspremnik GW2 | 50 ml | 2 x 100 ml |
| Proteinaza K | 50 ml | 200 ml |
| Pufer za otapanje proteaze | 5 ml | 15 ml |
| Međuspremnik AE | 30 ml | 120 ml |
Skladištenje i stabilnost
- Proteinazu K treba čuvati na 2-8°C po dolasku.
- Short-term storage at room temperature (15-25°C) for up to 12 weeks does not affect its performance.
- Preostale komponente kita mogu se čuvati na sobnoj temperaturi (15-25°C) and remain stable for at least 18 mjeseci pod ovim uvjetima.
Vodič za kupnju
| Ime | MAČKA BR | Količina uzorka | Leukocyte protocol* | Colum type | Volumen eluacije | Average yield | Vrijeme po vožnji |
| Hipure Blood DNK mini komplet | D3111 | 10-200μl | 1ml | 2ml stupca | ≥20μl | 5-9μg/200μl | ≤30 minuta |
| HiPure Tissue&Blood DNA Midi Kit | D3113 | 0.2-2ml | 10ml | 1.5ml stupca | ≥300μl | 20-40μg/1m | ≤80 minutes |
| HiPure Tissue&Krvni DNK Maxi Kit | D3115 | 3 -10ml | 10ml | 15ml stupca | ≥700μl | 20-40μg/1m | ≤90 minutes |
| HiPure Tissue&Blood DNA 96 Kit | D3117 | 1-200μl | 1ml | 96 bunar ploča | 3-8μg/200μl |
Recenzije
Još nema recenzija.