This product is suitable for extracting total RNA from 50-150mg conventional plantor fungal samples as well as samples rich in polyphenolic and polysaccharides. This kit is based on silica gel column purification technology, and the extraction process does not require the use of toxic phenol chloroform extraction. The entire extraction process only takes 20-30 minuta. This kit adopts DNA filtration technology, which can efficiently filter and remove DNA. The obtained RNA can be directly used for experiments such as RT-PCR, Northern Blot, poly A+ purification, zaštita nukleinske kiseline, i in vitro prijevod.
pojedinosti
Sadržaj kompleta
| Sadržaj | R415002 | D415003 |
| Vremena pročišćavanja | 50 Pripreme | 250 Pripreme |
| HiPure RNA Mini Columns | 50 | 250 |
| gDNA Filter Columns | 50 | 250 |
| 2ml cijevi za prikupljanje | 100 | 500 |
| TCEP (1M) | 0.29 g | 5 x 0.29 g |
| Buffer EP | 1.0 ml | 5.0 ml |
| Buffer PSL | 50 ml | 250 ml |
| Međuspremnik RW1 | 50 ml | 250 ml |
| Međuspremnik RW2* | 20 ml | 2 x 50 ml |
| Voda bez RNaze | 10 ml | 30 ml |
Skladištenje i stabilnost
Except TCEP, the kit components can be stored dry at room temperature (15–25°C) i stabilni su najmanje 18 mjeseci pod ovim uvjetima. After receiving the product, it is recommended to store TCEP (dry powder) at -20-8°C. At low temperatures, Buffer PSL may form precipitates, dissolve it completely by 55°C water bath.
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