Uvod
The HiPure Plant RNA Midi Kit provides fast purification of high-quality RNA from plants, cell, tissues, and yeast using silica-membrane spin columns with a binding capacity of 1mg RNA. There is no need for phenol / chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or precipitation with isopropanol or LiCl. RNA purified using the RaPure Total RNA Purification System can be used for applications such as RT-PCR, Northern blotting, poli A+ RNA (mRNA) pročišćavanje, zaštita od nukleaze, i in vitro prijevod.
pojedinosti
Tehnički podaci
| Značajke | Tehnički podaci |
| Glavne funkcije | Isolation total RNA from <1g simple plant sample (including plant sample containing starch) without chloroform |
| Prijave | RT-PCR, qPCR, Sjeverna hibridizacija, second generation sequencing, zaštita nukleinske kiseline, in vitro prijevod |
| Metoda pročišćavanja | Midi spin stupac |
| Tehnologija pročišćavanja | Silika tehnologija, Tehnologija filtracije DNA |
| Procesna metoda | Priručnik (centrifugiranje ili vakuum) |
| Vrsta uzorka | Plant leaves, roots, stems, fruits, fungi, itd. |
| Količina uzorka | 0.5-1 g |
| Volumen eluacije | ≥400μl |
| Vrijeme po vožnji | ≤40 minuta |
| Nosivi volumen tekućine po stupcu | 4ml |
| Prinos vezivanja kolone | 1mg |
Načelo
The Kit isolates total RNA from up to 1g plant tissue. A short workflow enables RNA isolation with genomic DNA removal in less than 40 min. Samples are first lysed and homogenized. The lysate is passed through a DNA Mini column, ethanol is added to the flow-through, and the sample is applied to a RNA column. RNA binds to the membrane and contaminants are washed away. High-quality RNA is eluted in as little as 400µl water using the Kit.
Prednosti
- Efficient removal of DNA – unique genomic DNA removal column without DNase treatment
- Visoka kvaliteta – ukupna RNA visoke čistoće može se izravno koristiti u raznim osjetljivim nizvodnim aplikacijama
- Brzo – medium isolation of several samples can be completed in 40 minuta
- Sef – no phenol chloroform extraction required
- Osjetljiv – RNA can be recovered at the level of PG
- Široki spektar – various types of plant samples can be processed by diversity of operating procedures
Sadržaj kompleta
| Sadržaj | R415202 | D415203 |
| Vremena pročišćavanja | 20 Pripreme | 100 Pripreme |
| HiPure DNA Midi Column II | 20 | 100 |
| HiPure RNA Midi Columns II | 20 | 100 |
| 15ml cijevi za prikupljanje | 40 | 200 |
| Buffer RLC | 120 ml | 550 ml |
| Buffer PRC1 | 120 ml | 550 ml |
| Međuspremnik RW1 | 70 ml | 400 ml |
| Međuspremnik RW2* | 20 ml | 100 ml |
| Voda bez RNaze | 30 ml | 120 ml |
Skladištenje i stabilnost
The Kit can be stored dry at room temperature (15-25°C) i stabilni su najmanje 18 mjeseci pod ovim uvjetima. Provjerite jesu li svi puferi na sobnoj temperaturi kada se koriste. During shipment, crystals or precipitation may form in the Buffer RLC/PRC1. Dissolve by warming buffer to 37°C.
Recenzije
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