HiPure HP Plant RNA Mini Kit combines gvanidin isothiocyanate lysing and silica gel membrane purification technology to simplify total RNA extraction. Ultracentrifugation in CsCl purification and LiCl / ethanol precipitation are not required. The kit uses DNase digestion to completely remove DNA. It is suitable for extracting up to 100μg of total RNA (Uključujući miRNA) from plant samples less than 200mg. Several samples can be extracted within 40 minuta. The purified RNA can be directly used for RT-PCR, fluorescent quantitative RT-PCR, Sjeverna hibridizacija, second generation sequencing, itd.
pojedinosti
Tehnički podaci
| Značajke | Tehnički podaci |
| Glavne funkcije | Izolacija Ukupna RNA (include miRNA) iz <200mg difficult-to-extract plant samples (use low toxicity chloroform substitutes) |
| Prijave | RT-PCR, qPCR, Sjeverna hibridizacija, second generation sequencing, zaštita nukleinske kiseline, in vitro prijevod |
| Metoda pročišćavanja | Mini spin stupac |
| Tehnologija pročišćavanja | Silika tehnologija |
| Procesna metoda | Priručnik (centrifugiranje ili vakuum) |
| Vrsta uzorka | Hard-to-extraction plant samples such as fruit and seed, grape leaves, tea |
| Količina uzorka | ≤200 mg |
| Volumen eluacije | ≥30μl |
| Vrijeme po vožnji | 1-24 samples within 30 minuta |
| Nosivi volumen tekućine po stupcu | 800µl |
| Prinos vezivanja kolone | 100µg |
Načelo
This kit uses glass fiber filter membrane purification technique, and only requires simple combination-washing-elution steps. The sample is lysed and homogenized in the solution containing guanidine salt, ethanol is added to provide appropriate binding conditions, and transferred to the purification column for centrifugation. Up to 100µg of RNA can be selectively bound to the membrane, pollutants are efficiently washed off after three times of washing, and finally the purified RNA is eluted by RNase Free Water.
Prednosti
- Completely remove DNA by using of DNase
- Visoka kvaliteta – one-step RNA extraction reagent combined with silica gel column can obtain the highest concentration
- Brzo – može se izdvojiti nekoliko uzoraka 40 minutes by column method
- Osjetljiv – RNA can be recovered at the level of PG
- Široki spektar – various types of plant samples can be processed by diversity of operating procedures
Sadržaj kompleta
| Sadržaj | R416502 | D416503 |
| Vremena pročišćavanja | 50 Pripreme | 250 Pripreme |
| HiPure RNA Mini Columns | 50 | 250 |
| 2ml cijevi za prikupljanje | 100 | 250 |
| DNaza I | 600 μl | 5 x 600 μl |
| DNase pufer | 6 ml | 30 ml |
| Međuspremnik PAL | 60 ml | 270 ml |
| Buffer GXP2* | 20 ml | 100 ml |
| Buffer BDP | 60 ml | 270 ml |
| Međuspremnik RW1 | 50 ml | 250 ml |
| Međuspremnik RW2* | 20 ml | 2 x 50 ml |
| Voda bez RNaze | 10 ml | 30 ml |
Skladištenje i stabilnost
DNase I should be stored at -20-8°C upon arrival. Preostale komponente kita mogu se čuvati na sobnoj temperaturi (15-25°C) i stabilni su najmanje 18 mjeseci pod ovim uvjetima. Cijeli komplet može se čuvati na 2-8°C, ali u ovom slučaju pufere treba ponovno otopiti prije upotrebe. Provjerite jesu li svi puferi na sobnoj temperaturi kada se koriste.
Recenzije
Još nema recenzija.