This product provides fast and easy methods for purification of total DNA for reliable PCR and southern blotting. Total DNA can be purified from bacterial culture, body fluids, food and fermentation.
pojedinosti
Tehnički podaci
| Značajke | Tehnički podaci |
| Glavne funkcije | Isolation bacterial DNA from cultures, food and other samples |
| Prijave | PCR, southern blot and enzyme digestion, itd. |
| Metoda pročišćavanja | Mini spin stupac |
| Tehnologija pročišćavanja | Silika tehnologija |
| Procesna metoda | Priručnik (centrifugiranje ili vakuum) |
| Vrsta uzorka | Culture medium, swab, parasitic blood, tissue,sputum, itd. |
| Količina uzorka | Bacterial culture medium: 0.5-2 ml
Tissue samples: 50-100 mg Puna krv / cell suspension: 0.5-1 ml Viscous secretion: 0.1-1 g |
| Volumen eluacije | ≥30μl |
| Vrijeme po vožnji | ≤40 minuta |
| Nosivi volumen tekućine po stupcu | 800μl |
| Prinos vezivanja kolone | 100μg |
Načelo
Ovaj proizvod se temelji na pročišćavanju kolonom silicijevog dioksida. Uzorak se lizira i probavlja lizatom i proteazom, DNK se oslobađa u lizat. Prebaciti u adsorpcijsku kolonu. Nukleinska kiselina se adsorbira na membrani, while proteinis not adsorbed and is removed with filtration. Nakon pranja proteina i drugih nečistoća, Nukleinska kiselina je konačno eluirana puferom s malo soli (10mmTris, pH 9,0, 0.5mm EDTA). This product carries 0.1-0.2mm acid glass beads, which can improve the wall-breaking effect of lysozyme resistant bacteria, the success rate and DNA yield through physical bead grinding.
Prednosti
- Brzo – može se izdvojiti nekoliko uzoraka 40 minuta (after digestion)
- Visoka čistoća – purified DNA can be directly used in various downstream applications
- Dobra ponovljivost – silica technology can obtain ideal results every time
- Visoki oporavak – DNA can be recovered at the level of PG
- Široki spektar – it can deal with a variety of bacteria, including Gram-positive bacteria that are difficult to disrupt
- Sufficient components – this kit has carried lysozyme, protease K, RNaza A and glass beads
Sadržaj kompleta
| Sadržaj | D314602 | D314603 |
| Vremena pročišćavanja | 50 Pripreme | 250 Pripreme |
| Hipure DNA Mini Columns I | 50 | 2 x 125 |
| 2ml cijevi za prikupljanje | 50 | 2 x 125 |
| Glass Beads (0.1~0.2mm) | 20 g | 100 g |
| Buffer P1 | 20 ml | 100 ml |
| Buffer DL | 15 ml | 80 ml |
| Buffer GW1 | 22 ml | 88 ml |
| Međuspremnik GW2 | 12 ml | 50 ml |
| Lizocim | 60 mg | 300 mg |
| Proteinaza K | 24 mg | 120 mg |
| Pufer za otapanje proteaze | 5 ml | 15 ml |
| Međuspremnik AE | 15 ml | 60 ml |
Skladištenje i stabilnost
Lysozyme and Proteinase K should be stored at 2-8°C upon arrival. Međutim, kratkotrajno skladištenje (do 12 tjedni) na sobnoj temperaturi (15-25°C) ne utječe na njihovu izvedbu. Preostale komponente kita mogu se čuvati na suhom na sobnoj temperaturi (15-25°C) i stabilni su najmanje 18 mjeseci pod ovim uvjetima.
Recenzije
Još nema recenzija.