Uvod
Proizvod je jezgrena sirovina za ekstrakciju nukleinskih kiselina nakon strogog probira i kontrole kvalitete. Guanidine hydrochloride is a powerful denaturantor ionizing agent, which can promote the solubility of hydrophobic molecules and denature proteins. It is also an effective denaturant and inactivator of avariety of enzymes and proteins. In 6 M guanidine hydrochloride solution, the ordered “secondary structure” (maintained by intermolecular non covalent interactions, such as hydrogen bonds and hydrophobic bonds) of almost all proteins is destroyed and the peptide chain is stretched. The tertiary structure has changed. U procesu ekstrakcije nukleinske kiseline, guanidine hydrochloride is often used as cleaning solution (> 3M), binding solution (4-6M) for protein removal, and lysate for genomic DNA and total RNA extraction. Different from guanidine isothiocyanate, guanidine hydrochloride has low absorbance in A260, A280 and A230, and the ratio of nucleic acid product A260 /230 is more ideal.
pojedinosti
Tehnički podaci
| Značajke | Specifikacija | |
| Basic content | Preporučena prijava | Plasmid extraction, lysis Solution or cleaning solution |
| CAS brojevi | 50-01-1 | |
| Formula | CH5N3.HCL | |
| Molekularna masa | 95.53 | |
| Content | 99.5% | |
| Nivo | Molekularna biologija | |
| Izgled | White crystal, will harden for a long time | |
| Transportation conditions | Room temperature | |
| Uvjeti očuvanja | Room temperature, dry, dark | |
| Impurity parameters | Moisture | ≤0.3% |
| Ash | ≤0.02% | |
| Water insoluble matter | ≤0.02% | |
| Ammonium | ≤0.05% | |
| Ammonium dicyanide | ≤0.1% | |
| Ammonium cyanuric chloride | ≤0.01% | |
| Iron | ≤0.1PPM | |
| UV absorption value | Absorbance value @ 230 (6M) | ≤0.4 |
| Absorbance value @ 260 (6M) | ≤0.04 | |
| Absorbance value @ 280 (6M) | ≤0.01 | |
| Absorbance value @ 320 (6M) | ≤0.01 | |
| Nucleic acid extraction related | Plasmid extraction test | Adopt |
| Blood DNA extraction test | Adopt | |
| DNase test (2M) | Nije otkriveno | |
| RNase test (2M) | Nije otkriveno | |
| Purity of saturated solution (8M) | The 8m mother liquor is heated and dissolved. After dissolution, the insoluble matter is filtered with 3 layers of rapid filter paper. | |
| PH value (6M) | 5.0-5.5 |
Informacije o naručivanju
| Mačka.no. | Naziv proizvoda | Paket |
| C11101 | Guanidine Hydrochloride
(Molekularna biologija) |
5Kg |
| C11102 | 25Kg (5KG/Bag, 5Bags/Bucket) |
Recenzije
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