This product is designed for purification of high-molecular-weight genomic or mitochondrial DNA from a variety of tissue and culture cells. The convenient, scalable purification procedure removes contaminants and enzyme inhibitors such as proteins and divalent cation, and purified DNA is ready for immediate use in sensitive downstream applications or for archiving.
Detalles
Especificaciones
| Características | Especificaciones |
| Funciones principales | Isolation total DNA from tissue using economic salt out method |
| Aplicaciones | PCR, digestión enzimática, Southern hybridization,etc.. |
| Tecnología de purificación | Salting out method |
| método de proceso | Manual (centrifugación o vacío) |
| Tipo de ejemplo | Tejido animal |
| Cantidad de muestra | Unlimited |
| Volumen de elución | ≥100μl |
| Tiempo por carrera | Variation with sample amount |
Principios
Cells are lysed with ananionic detergent in the presence of a DNA stabilizer. The DNA stabilizer limits the activity of intracellular DNases and also DNases found elsewhere in the environment. RNA is then removed by treatment with an RNA digesting enzyme. Other contaminants, such as proteins, are removed by salt precipitation. Finalmente, the genomic DNA is recovered by precipitation with alcohol and dissolved in Buffer TE. Purified DNA typically has an A260/A280 ratio between 1.7 y 1.9, and is up to 200 kb in size. The DNA can be safely stored at 2-8°C, -20°C, or -80°C.
Ventajas
- Seguro – without phenol chloroform extraction
- Environment friendly – the reagents used are safe, non-toxic and without pollution
- High molecular weight – the molecular weight of genomic DNA is about 50-150kb
- Alta pureza – the purified DNA has A260/280=1.8-1.9, A260/230=2.0-2.5
- Unlimited sample size – solution type operation, sample volume can be adjusted at will
- Cost performance – the most economical nucleic acid extraction technology
Contenido del kit
| Contenido | D331201 | D331202 | D331203 |
| Tiempos de purificación | 1 gramo | 5 gramo | 50 gramo |
| Proteinasa K | 330 l | 1.8 ml | 18 ml |
| Buffer WTL | 33 ml | 160 ml | 2 X 800 ml |
| Buffer PPS | 12 ml | 55 ml | 500 ml |
| Solución de ARNasa | 330 l | 1.8 ml | 18 ml |
| Buffer TE | 12 ml | 60 ml | 200 ml |
Almacenamiento y estabilidad
RNase Solution should be stored at 2-8°C upon arrival. Sin embargo, almacenamiento a corto plazo (hasta 24 semanas) a temperatura ambiente (15-25°C) no afecta su rendimiento. Los componentes restantes del kit se pueden almacenar secos a temperatura ambiente. (15-25°C) y son estables durante al menos 18 meses en estas condiciones. Todo el kit se puede almacenar a 2-8°C., pero en este caso los tampones deben volver a disolverse antes de su uso.. Asegúrese de que todos los tampones estén a temperatura ambiente cuando se utilicen..
Reseñas
Aún no hay reseñas.