Introducción
- Extracts DNA from various sources: tejido, células, sangre, saliva, hisopos, manchas de sangre, semen, y otras muestras clínicas.
- Fast and easy to use.
- Purified DNA can be used for PCR, qPCR, Mancha del Sur, viral DNA testing, y más.
Especificaciones
| Características | Especificaciones |
| Funciones principales | Isolation of total DNA from 1-100ul blood, FPE, tejido, y otras muestras |
| Aplicaciones | Secuenciación de segunda generación, PCR, PCR en tiempo real, etc.. |
| Método de purificación | Monodisperse magnetic beads |
| Tecnología de purificación | Tecnología de cuentas magnéticas |
| método de proceso | Manual o automático |
| Tipo de ejemplo | Sangre anticoagulante, sangre concentrada, capa leucocitaria, linfocitos, y células cultivadas |
| Cantidad de muestra | Body fluid : 10 – 200µl, Tejido : ≤10mg |
| Producir | 10ng – 15µg |
| Volumen de elución | 80 -100µl |
| Tiempo por carrera |
Principio
- Utilizes high-binding magnetic particles for purification, ensuring efficient DNA isolation.
- The sample undergoes lysis and digestion facilitated by lysate and Protease, releasing DNA into the solution.
- Magnetic particles and binding solution are introduced, causing DNA to adhere to particle surfaces while impurities such as proteins are left unbound.
- Wash with a specialized solution to remove proteins and impurities, followed by ethanol wash to eliminate salts.
- DNA is ultimately eluted using an Elution Buffer, providing purified DNA ready for downstream applications.
Advantage
- High binding capability – ideal for processing DNA-rich samples like whole blood, capa leucocitaria, and concentrated blood.
- Exceptional purity – features carboxyl magnetic beads with minimal surface adsorption, ensuring superior purity.
- Versatile – applicable across a wide range of clinical samples, offering general utility.
Contenido del kit
| Contenido | IVD3101 |
| Tiempos de purificación | 200 |
| Unir partículas | 4.5 ml |
| Proteinasa K | 90 mg |
| Tampón de disolución de proteasa | 10 ml |
| ATL de búfer | 60 ml |
| Amortiguador AL | 60 ml |
| Tampón BD* | 20 ml |
| Búfer bxw1* | 110 ml |
| Tampón de elución | 30 ml |
Almacenamiento y estabilidad
- Proteinase K and MagBind Particles should be stored at 2–8°C upon receipt.
- Almacenamiento a corto plazo (hasta 18 semanas) a temperatura ambiente (15–25°C) does not compromise their performance.
- The remaining components can be stored dry at room temperature (15–25°C) y permanecer estable por al menos 18 meses en estas condiciones.
