Microkit de ácido nucleico circulante HiPure

Microkit de ácido nucleico circulante HiPure, is designed to efficiently purify short nucleic acids (<1000pb) from human biological fluids.

• Target Molecules:
  • Tumor-derived cell-free DNA fragments (cfdna) and mRNA in blood
  • Ácidos nucleicos fetales en sangre materna
  • Free-circulating cell-free RNA (cfRNA)
  • Viral nucleic acids
• Compatibilidad de muestra:
  • Plasma humano
  • Suero humano
  • Orina humana
  • Las muestras pueden estar frescas o congeladas (Una sola vez)
• Purificación eficiente: The kit isolates short nucleic acids present in biofluids.
• Alta pureza: Purified nucleic acids are free of proteins, nucleases, y otros contaminantes.
• Aplicaciones aguas abajo: The eluted nucleic acids are ready for use in various downstream analyses, incluido:
  • Amplification reactions (p.ej., PCR)
  • Long-term storage (-30°C to -15°C)

Beneficios:

• Suitable for Short Nucleic Acids: Specifically designed to capture short fragments often found in biofluids.
• Compatibilidad de muestra amplia: Funciona de manera efectiva con varios biofluidos humanos.
• High-Quality Results: Delivers purified nucleic acids free of impurities, ensuring reliable downstream applications.
• Convenient Storage: Purified nucleic acids can be stored for long-term use.

This kit empowers researchers studying circulating nucleic acids in various fields, incluyendo oncología, Pruebas prenatales no invasivas (Sorbo), and viral diagnostics.

Especificaciones

Principio

This product offers a fast and efficient method for purifying DNA using silica column chromatography. Here’s a breakdown of the process:

1. Lisis y digestión:

• The sample is treated with a lysate solution and protease to break open cells and degrade proteins. This releases DNA into the solution.

2. Unido a la membrana de sílice:

• The lysate containing the DNA is transferred to an adsorption plate or filtration column. The silica membrane in these tools selectively binds DNA molecules due to favorable interactions, while proteins and other impurities pass through.

3. Lavado:

• Wash buffers are passed through the column, removing unbound proteins and cellular debris that didn’t adhere to the silica.

4. Elución:

• A low-salt buffer is used to elute the purified DNA from the silica membrane. This disrupts the interaction between DNA and the silica, allowing collection of purified DNA in a separate tube.

Este método proporciona una herramienta valiosa para los investigadores que trabajan en varios campos que requieren ADN purificado para el análisis., such as PCR, transferencia del sur, and next-generation sequencing.

Ventajas

• Purificación eficiente: Silica column technology offers a streamlined process for isolating DNA from complex biological samples.
• ADN de alta calidad: The selective binding properties of the silica membrane ensure the purification of high-purity DNA, suitable for various downstream applications.
• Flujo de trabajo simplificado: The protocol involves minimal handling steps and centrifugation for user-friendly and efficient extraction.

Contenido del kit

Almacenamiento y estabilidad

Optimal Performance:

• Store Proteinase K and Carrier RNA at 2-8°C (refrigerador) upon arrival for best results.

Acceptable Short-Term Storage:

• Proteinase K and Carrier RNA can be stored for up to 12 weeks at room temperature (15-25°C) without affecting their performance.

Componentes del kit restantes:

• Store all other components of the kit dry at room temperature (15-25°C).
• Son estables por al menos 18 meses en estas condiciones.

Important Notes:

• If storing the entire kit at 2-8°C, ensure buffers are redissolved before use.
• Always warm all buffers to room temperature before using them for optimal performance.

By following these storage guidelines, you can ensure the longevity and effectiveness of your DNA extraction kit.