Einführung
This product is specially designed for stool RNA-Extraktion. The kit is suitable for extracting high-purity microbial or host cell RNA from ≤ 0.15g stool samples. The purified RNA can be directly used in RT-PCR and Northern hybridization.
Einzelheiten
Prinzip
The Kit combines the speed and efficiency of silica-based technology with the convenient handling of magnetic particles for purification of total RNA. Samples are lysed and RNA is purified from lysates in one step through its binding to the silica surface of the particles in the presence of a chaotropic salt. The particles are separated from the lysates using a magnet and DNA is removed by treatment with RNase-free DNase. The magnetic particles are efficiently washed, and RNA is eluted in RNase-free water.
Inhalt des Kits
| Inhalt | R662601 | R662602 | R662603 |
| Reinigungszeiten | 48 Vorbereitungen | 96 Vorbereitungen | 5 X 96 Vorbereitungen |
| MagPure RNA Particles | 1.7 ml | 4.0 ml | 18 ml |
| DNase I | 600 μl | 2 X 600 μl | 10 X 600 μl |
| DNase Buffer | 30 ml | 40 ml | 200 ml |
| Puffer spl | 30 ml | 60 ml | 270 ml |
| Buffer PHC | 30 ml | 60 ml | 270 ml |
| Buffer MCB* | 18 ml | 30 ml | 150 ml |
| Buffer MW1* | 44 ml | 66 ml | 2 X 220 ml |
| Puffer RW2* | 20 ml | 50 ml | 2 X 100 ml |
| RNase-freies Wasser | 10 ml | 30 ml | 120 ml |
| 2ML Perlenröhrchen | 48 | 96 | 5 X 96 |
Lagerung und Stabilität
MagPure RNA Particles should be stored at 2–8°C upon arrival. DNase I should be stored at -20°C. Jedoch, kurzfristige Lagerung (DNase I up to 1 Wochen, MagPure RNA Particles up to 8 Wochen) bei Raumtemperatur (15–25°C) hat keinen Einfluss auf ihre Leistung. Die restlichen Kit-Komponenten können bei Raumtemperatur gelagert werden (15–25°C) und sind mindestens stabil 18 months under these
Rezensionen
Es gibt noch keine Rezensionen.