Einführung
Magnetic bead nucleic acid purification technology uses nano or micron superparamagnetic material as the matrix, generally black ferric oxide or yellowish-brown ferric oxide as the magnetic material. The surface of the bead is coated with appropriate functional groups, which can adsorb nucleic acid. Magnetic beads are commonly used for nucleic acids, containing carboxyl groups, hydroxyl groups, or silicon groups. Silicon-based magnetic beads are the most common, and their principle of adsorbing nucleic acid is consistent with the classical glass milk purification technology or glass fiber filter membrane purification method. Magpure particle is a kind of polydisperse fast-speed silica magnetic beads. The core is ferric oxide, accounting for 50%, and the surface coating is silica, accounting for 50%. The product can be used for plasmid extraction, gel DNA recovery, product purification, genomic DNA and RNA-Extraktion, and viral nucleic acid extraction.
Einzelheiten
Spezifikationen
| Merkmale | Spezifikationen |
| Konzentration | 70 mg/ml |
| Aussehen | Suspension of yellowish-brown particles |
| Surface functional group | Si-OH, Silanol |
| Dispersibility | Polydisperse Amorphous |
| Particle size | 0.2-2 μm |
| Aufbewahrungsbedingungen | Zimmertemperatur, valid for up to 2 Jahre.
It is recommended to store at 2-8°C to prevent microbial growth. |
| Magnetic response speed | ~60 seconds |
| Settling velocity | >10 Protokoll |
| High salt-mediated binding | >2M Guanidin isothiocyanate, DNA recovery up to 80% |
| Alcohol mediated binding | 2M guanidine hydrochloride/isopropanol (30%), and the recovery of DNA / RNA was as high as 85% |
| PEG8000 mediated binding | The recovery of DNA/RNA was up to 85% |
| DNase/RNase | Nicht erkannt |
| DNA residue | <1 ppm |
| Empfohlene Anwendung | Plasmid extraction, gel DNA recovery, viral nucleic acid isolation |
Prinzip
Highsalt mediated binding: in the solution containing 2-4M guanidine isothiocyanate, Magpure particles can selectively recover DNA molecules, and impurities such as protein polysaccharides are not adsorbed.
Alcohol-mediated binding: in the solution containing guanidine salt and alcohol (>25%), Magpure particles can selectively recover DNA/RNA molecules, and proteins and other impurities are not adsorbed.
After biological samples are treated with a digestive solution or lysis Buffer, DNA/RNA is released from cells, organelles, and protein complexes (ribosomes and nucleosomes) into reagents. After Magpure particles and binding solution are added, DNA/RNA is adsorbed to the surface of Magpure particles to form the DNA/RNA bead complex. Under the action of the magnetic field, the magnetic beads are separated and collected, and the impurities such as protein are removed with the waste liquid. After two or three steps of further cleaning, the DNA/RNA magnetic bead complex is resuspended in sterilized water or TE buffer, and the DNA/RNA falls off from the surface of the magnetic beads, to achieve the purpose of purification.
Bestellinformationen
| Katze nein. | Produktname | Paket |
| C14110 | MagPure-Partikel N | 100 ml |
| C14111 | MagPure-Partikel N | 400 ml |
| C14112 | MagPure-Partikel N | 3 X 400 ml |
| C14113 | MagPure-Partikel N | 10 X 400 ml |
Kaufratgeber
| Merkmale | Elsträgerpartikel | MagPure-Partikel N | Elsträgerpartikel g | MagPure Particles F | Partikel binden |
| Cat.No. | C1410 | C1411 | C1412 | C1414 | C1413 |
| Konzentration | 100mg/ml | 70mg/ml | 40mg/ml | 50mg/ml | 10mg/ml |
| Form | Amorphous and Porous | Amorphous and Porous | Porous | Amorphous | Nonporous |
| Surface function | Si-OH, Silica Beads | Si-OH, Silica Beads | Si-OH, Silica Beads | Si-OH, Silica Beads | COOH, Carboxyl Beads |
| Dispersion | Polydisperse | Polydisperse | Monodisperse | Monodisperse | Monodisperse |
| Particle Size | 1.5-5μm | 0.2-2μm | 1-1.5μm | 0.2-1.5μm | 0.8-1μm |
| Farbe | Black | Yellowish Brown | Dark Brown | Dark Brown | Yellowish Brown |
| Magnetic response | 15-30S | ~60s | ~30s | 20S | 120S |
| Settling Time (1ml) | >5Mindest | >10Mindest | >3Mindest | >3Mindest | >2H |
| Usage (0.2ml Sample) | 20μl | 20μl | 20-30μl | 20-30μl | 20-30μl |
| DNA Recover Rate (only 4M GITC) | >80% | >80% | >80% | >80% | 0 |
| DNA Recover Rate (10% PEG8000/NaCl) | >85% | >85% | >85% | >85% | >90% |
| Recommended Use |
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- The MagPure magnetic-particle technology combines the speed and efficiency of silica-based DNA purification with the convenient handling of magnetic particles. DNA binds to the silica surface of the magnetic particles in the presence of a chaotropic salt. DNA bound to the particles is then efficiently washed, considerably improving the purity of DNA. High-quality DNA is eluted. The automated purification procedure completely removes enzymes, Nukleotide, and other contaminants and inhibitors. Purified DNA is suitable for direct use in downstream applications, such as sequencing and microarray analysis.
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