Einführung
- Magnetic bead nucleic acid purification technology utilizes nano or micron superparamagnetic material as the matrix, often using black or yellowish-brown ferric oxide.
- The surface of the beads is coated with functional groups such as carboxyl, hydroxyl, or silicon groups, which facilitate nucleic acid adsorption.
- Silicon-based magnetic beads are the most common, working similarly to classical glass milk or glass fiber filter membrane purification methods.
- Magpure particles are polydisperse silica magnetic beads, comprising 50% ferric oxide at the core and 50% silica on the surface.
- This product serves various purposes including plasmid extraction, gel DNA recovery, product purification, genomic DNA and RNA-Extraktion, as well as viral nucleic acid extraction.
Spezifikationen
| Merkmale | Spezifikationen |
| Konzentration | 100 mg/ml |
| Aussehen | Suspension of black particles |
| Surface functional group | Si-OH, Silanol |
| Dispersibility | Polydisperse Amorphous |
| Particle size | 1.5-5 μm |
| Aufbewahrungsbedingungen | Zimmertemperatur, valid for up to 2 Jahre.
It is recommended to store at 2-8°C to prevent microbial growth. |
| Magnetic response speed | 15-30 Sekunden |
| Settling velocity | >5 Protokoll |
| High salt-mediated binding | >2M Guanidin isothiocyanate, DNA recovery up to 80% |
| Alcohol mediated binding | 2M guanidine hydrochloride/isopropanol (30%), and the recovery of DNA / RNA was as high as 85% |
| PEG8000 mediated binding | The recovery of DNA/RNA was up to 85% |
| DNase/RNase | Nicht erkannt |
| DNA residue | <1 ppm |
| Empfohlene Anwendung | Plasmid extraction, gel DNA recovery, genomische DNA-Extraktion, and RNA extraction. |
Prinzip
- High salt mediated binding: Magpure particles can selectively recover DNA molecules in solutions containing 2-4M guanidine isothiocyanate, while impurities like protein polysaccharides are not adsorbed.
- Alcohol-mediated binding: Magpure particles can selectively recover DNA/RNA molecules in solutions containing guanidine salt and alcohol (>25%), with proteins and other impurities not being adsorbed.
- After treating biological samples with a digestive solution or lysis buffer, DNA/RNA is released into the reagents from cells, organelles, and protein complexes (ribosomes and nucleosomes).
- Upon adding Magpure particles and binding solution, DNA/RNA adheres to the surface of the particles, forming the DNA/RNA bead complex.
- The magnetic field then separates and collects the magnetic beads, removing impurities such as protein with the waste liquid.
- Following two or three additional cleaning steps, the DNA/RNA magnetic bead complex is resuspended in sterilized water or TE buffer.
- DNA/RNA falls off from the surface of the magnetic beads, achieving purification.
Bestellinformationen
| Katze nein. | Produktname | Paket |
| C14100 | Elsträgerpartikel | 100 ml |
| C14101 | Elsträgerpartikel | 400 ml |
| C14102 | Elsträgerpartikel | 3 X 400 ml |
| C14103 | Elsträgerpartikel | 10 X 400 ml |
Kaufratgeber
| Merkmale | Elsträgerpartikel | MagPure-Partikel N | Elsträgerpartikel g | MagPure Particles F | Partikel binden |
| Cat.No. | C1410 | C1411 | C1412 | C1414 | C1413 |
| Konzentration | 100mg/ml | 70mg/ml | 40mg/ml | 50mg/ml | 10mg/ml |
| Form | Amorphous and Porous | Amorphous and Porous | Porous | Amorphous | Nonporous |
| Surface function | Si-OH, Silica Beads | Si-OH, Silica Beads | Si-OH, Silica Beads | Si-OH, Silica Beads | COOH, Carboxyl Beads |
| Dispersion | Polydisperse | Polydisperse | Monodisperse | Monodisperse | Monodisperse |
| Particle Size | 1.5-5μm | 0.2-2μm | 1-1.5μm | 0.2-1.5μm | 0.8-1μm |
| Farbe | Black | Yellowish Brown | Dark Brown | Dark Brown | Yellowish Brown |
| Magnetic response | 15-30S | ~60s | ~30s | 20S | 120S |
| Settling Time (1ml) | >5Mindest | >10Mindest | >3Mindest | >3Mindest | >2H |
| Usage (0.2ml Sample) | 20μl | 20μl | 20-30μl | 20-30μl | 20-30μl |
| DNA Recover Rate (only 4M GITC) | >80% | >80% | >80% | >80% | 0 |
| DNA Recover Rate (10% PEG8000/NaCl) | >85% | >85% | >85% | >85% | >90% |
| Recommended Use |
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- MagPure magnetic-particle technology combines the speed and efficiency of silica-based DNA purification with the convenience of magnetic particles.
- DNA binds to the silica surface of the magnetic particles in the presence of a chaotropic salt.
- Bound DNA is efficiently washed, significantly enhancing DNA purity.
- High-quality DNA is eluted from the magnetic particles.
- The automated purification procedure removes enzymes, Nukleotide, and other contaminants and inhibitors completely.
- Purified DNA is ready for direct use in downstream applications, including sequencing and microarray analysis.
Rezensionen
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