MagPure A3 Features:
- Technology Base:
- Utilizes Magen’s solid-phase paramagnetic bead technology for the purification of PCR amplicons.
- High-Throughput Compatibility:
- Designed for high-throughput applications, facilitating efficient processing of multiple samples.
AmPure Technology:
- Selektive Bindung:
- Features an optimized buffer that selectively binds PCR amplicons of 100bp and larger to paramagnetic beads.
- Verunreinigungsentfernung:
- Efficiently removes excess primers, Nukleotide, salts, and enzymes through a simple washing procedure.
- Purity of PCR Product:
- Delivers PCR products that are essentially free of contaminants, enhancing downstream application reliability.
Einzelheiten
Spezifikationen
| Merkmale | Spezifikationen |
| Hauptfunktionen | Selectively recover DNA from PCR products and enzymatic reaction solution (Replace Beckmen or agencourt AmPure) |
| Anwendungen | Sequenzierung, gene chip and qPCR, usw. |
| Reinigungstechnologie | Magnetperlen-Technologie |
| Prozessmethode | Handbuch (Zentrifugation oder Vakuum) |
| Beispielstyp | PCR-Produkte, enzymatic reaction solution |
| Probenmenge | Appropriate |
| Elutionsvolumen | ≥10μl |
| Operationszeit | ≤ 50 Minuten |
Prinzip
Product Purification Method:
- High Binding Magnetic Particle Method:
- Utilizes high binding magnetic particles for purification.
- PCR Amplicon Binding:
- PCR amplicons are mixed with MagPure A3.
- DNA fragments of 100bp and larger bind to magnetic beads.
- Verunreinigungsentfernung:
- Excess primers, Nukleotide, salts, and enzymes are removed via a simple washing procedure.
- DNA -Elution:
- Purified DNA is eluted using Elution Buffer or Water.
Vorteile
- Wide application – DNA-Produkte, enzyme digestion system, PCR reaction
- Hohe Genesung – suitable for 100bp-20kb fragments
- Hohe Reinheit – effectively remove dNTP, primer, primer dimer, salt ion and other impurities
- Strong applicability – manual operation or automatic workstation
Inhalt des Kits
| Inhalt | BXP-5 | BXP-50 | BXP-500 |
| Elstern A3 | 5 ml | 50 ml | 500 ml |
Lagerung und Stabilität
Storage and Handling Instructions:
- Lagerbedingungen:
- Store MagPure A3 at 2-8°C upon arrival.
- Stable for up to 18 Monate unter diesen Bedingungen.
- Short-term Storage:
- Kurzfristige Lagerung (bis zu 12 Wochen) bei Raumtemperatur (15-25°C) does not affect performance.
- Reagent Preparation:
- Shake the reagent well before use to ensure homogeneity.
- The reagent should appear homogenous and consistent.
DO NOT FREEZE.
Rezensionen
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