Hipure Plasmid EF Mini Kit

Einführung

HiPure Plasmid EF Mini Kit Overview:

1. Technology Integration:
   • Combines HiPure technology with Magen’s innovative Endotoxin Removal Technology (ETR) to ensure high-quality plasmid DNA-Reinigung.
2. Endotoxin Management:
   • Features a specially formulated buffer designed to prevent endotoxins from binding to the HiPure matrix, significantly reducing endotoxin levels in the final DNA product.
3. Application Suitability:
   • Provides plasmid DNA ideal for eukaryotic transfection and in vitro experiments, especially where low endotoxin levels are critical.
4. Impact of Endotoxin Contamination:
   • Endotoxin contamination can drastically lower transfection efficiencies, particularly in endotoxin-sensitive cell lines.
5. Clinical Relevance:
   • In contexts such as gene therapy, controlling endotoxin levels is crucial due to the potential for serious adverse effects like fever, endotoxin shock syndrome, and negative impacts on in vitro transfection into immune cells.

Einzelheiten

Spezifikationen

Prinzip

The HiPure Plasmid procedure is based on alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt. The unique silica membrane used in the kit completely replaces glass or silica slurries for plasmid DNA minipreps. The procedure consists of 3 basic steps: Preparation and clearing of a bacterial lysate by alkaline method，then transfer the supernatant to column to bind DNA. Nach dem Waschen von Proteinen und anderen Verunreinigungen, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9,0, 0.5mm EDTA).

Vorteile

• Hohe Reinheit – purified plasmid can be directly used in sequencing, enzyme digestion and PCR, usw.
• Schnell – silica gel column purification is much faster than ion exchange
• Hohe Ausbeute – up to 70μg plasmid can be binded in one column
• Low endotoxin content – the obtained plasmid can be directly used in cell transfection and animal injection

Inhalt des Kits

Lagerung und Stabilität

Speicheranweisungen:

1. General Storage:
   • Kit components can be stored dry at room temperature (15–25°C).
   • Stabil für zumindest 18 Monate unter diesen Bedingungen.
2. Handling Precipitates:
   • Falls sich in den Puffern Ausfällungen bilden, warm at 37°C to dissolve.
3. Specific Storage for Buffer P1 with RNase A:
   • After addition of RNase A, Buffer P1 is stable for 6 months when stored at 2–8°C.

Kaufratgeber