Einführung
The HiPure Plant RNA Midi Kit provides fast purification of high-quality RNA from plants, Zelle, Gewebe, and yeast using silica-membrane spin columns with a binding capacity of 1mg RNA. There is no need for phenol / chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or precipitation with isopropanol or LiCl. RNA purified using the RaPure Total RNA Purification System can be used for applications such as RT-PCR, Northern Blot, poly A+ RNA (mRNA) Reinigung, nuclease protection, and in vitro translation.
Einzelheiten
Spezifikationen
| Merkmale | Spezifikationen |
| Hauptfunktionen | Isolation total RNA from <1g simple plant sample (including plant sample containing starch) without chloroform |
| Anwendungen | RT-PCR, qPCR, Nordhybridisierung, second generation sequencing, nucleic acid protection, in vitro translation |
| Reinigungsmethode | Midi Spin-Säule |
| Reinigungstechnologie | Silica-Technologie, DNA filtration technology |
| Prozessmethode | Handbuch (Zentrifugation oder Vakuum) |
| Beispielstyp | Plant leaves, roots, stems, fruits, fungi, usw. |
| Probenmenge | 0.5-1 G |
| Elutionsvolumen | ≥400μl |
| Zeit pro Lauf | ≤ 40 Minuten |
| Flüssigkeitstransportvolumen pro Säule | 4ml |
| Bindungsausbeute der Säule | 1mg |
Prinzip
The Kit isolates total RNA from up to 1g plant tissue. A short workflow enables RNA isolation with genomic DNA removal in less than 40 Mindest. Die Proben werden zunächst lysiert und homogenisiert. The lysate is passed through a DNA Mini column, ethanol is added to the flow-through, and the sample is applied to a RNA column. RNA binds to the membrane and contaminants are washed away. High-quality RNA is eluted in as little as 400µl water using the Kit.
Vorteile
- Efficient removal of DNA – unique genomic DNA removal column without DNase treatment
- Gute Qualität – high purity total RNA can be directly used in various sensitive downstream applications
- Schnell – medium isolation of several samples can be completed in 40 Protokoll
- Sicher – keine Phenol-Chloroform-Extraktion erforderlich
- Sensitive – RNA kann auf der Ebene von PG wiederhergestellt werden
- Breites Spektrum – various types of plant samples can be processed by diversity of operating procedures
Inhalt des Kits
| Inhalt | R415202 | D415203 |
| Reinigungszeiten | 20 Vorbereitungen | 100 Vorbereitungen |
| HiPure DNA Midi Column II | 20 | 100 |
| HiPure RNA Midi Columns II | 20 | 100 |
| 15ml-Sammelröhrchen | 40 | 200 |
| Puffer-RLC | 120 ml | 550 ml |
| Buffer PRC1 | 120 ml | 550 ml |
| Puffer RW1 | 70 ml | 400 ml |
| Puffer RW2* | 20 ml | 100 ml |
| RNase-freies Wasser | 30 ml | 120 ml |
Lagerung und Stabilität
The Kit can be stored dry at room temperature (15-25°C) und sind mindestens stabil 18 Monate unter diesen Bedingungen. Stellen Sie sicher, dass alle Puffer bei der Verwendung Raumtemperatur haben. During shipment, crystals or precipitation may form in the Buffer RLC/PRC1. Dissolve by warming buffer to 37°C.
Rezensionen
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