The HiPure Fruit RNA Mini Kit provides fast purification of high-quality RNA from fruits, Zelle, Gewebe, and yeast using silica-membrane spin columns with a binding capacity of 100µg RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or precipitation with isopropanol or LiCl. RNA purified using the RaPure Total RNA Purification System can be used for applications such as RT-PCR, Northern Blot, poly A+ RNA(mRNA) Reinigung, nuclease protection and in vitro translation.
Einzelheiten
Spezifikationen
| Merkmale | Spezifikationen |
| Hauptfunktionen | Isolation total RNA from <150 mg fruit or seed using chloroform and Plantzol reagent |
| Anwendungen | RT-PCR, qPCR, Nordhybridisierung, Poly A purification, nucleic acid protection and in vitro translation translation |
| Reinigungsmethode | Mini Spin-Säule |
| Reinigungstechnologie | Silica-Technologie, DNA filtration technology |
| Prozessmethode | Handbuch (Zentrifugation oder Vakuum) |
| Beispielstyp | Simple plant, petal, peel, seed |
| Probenmenge | 50-150mg |
| Elutionsvolumen | ≥ 300 μl |
| Zeit pro Lauf | ≤15 minutes（1-24 samples） |
| Flüssigkeitstransportvolumen pro Säule | 800µl |
| Bindungsausbeute der Säule | 100µg |
Prinzip
The Kit isolates total RNA from up to 150mg plant tissue. A short workflow enables RNA isolation with genomic DNA removal in less than 15 Mindest. Die Proben werden zunächst lysiert und homogenisiert. The lysate is passed through a DNA Mini column, ethanol is added to the flow-through, and the sample is applied to an RNA column. RNA binds to the membrane and contaminants are washed away. High-quality RNA is eluted in as little as 300µl water using the Kit.
Vorteile
- Efficient DNA removal – one step RNA-Extraktion can effectively remove genomic DNA
- Gute Qualität – one step RNA extraction reagent combined with silica gel column can obtain the highest concentration
- Schnell – Es können mehrere Proben entnommen werden 15 minutes by column method
- Wide range – samples including animals, Pflanzen, Bakterien, Zellen, usw
- High output – large sample amount, and the yield can be up to 200μg
Inhalt des Kits
| Inhalt | R401402 | D401403 |
| Reinigungszeiten | 50 Vorbereitungen | 250 Vorbereitungen |
| HiPure RNA Mini-Säulen | 50 | 250 |
| 2ml-Sammelröhrchen | 50 | 250 |
| PlantZol Reagent | 60 ml | 200 ml |
| Buffer GXP | 30 ml | 150 ml |
| Puffer RW1 | 50 ml | 200 ml |
| Puffer RW2* | 20 ml | 2 X 50 ml |
| RNase-freies Wasser | 10 ml | 30 ml |
Lagerung und Stabilität
The Kit can be stored dry at room temperature (15–25°C) und sind mindestens stabil 18 Monate unter diesen Bedingungen. During shipment, crystals or precipitation may form in the Buffer RLC/PRC1. Dissolve by warming buffer to 37°C.
Rezensionen
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